Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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Our main goal was to test organisms’ resistance and susceptibility to bacteria‚ and whether the cell wall of bacteria exposed the membrane to pathogen or protect it from pathogens. I thought P. aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used
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not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not alter the equilibrium of a chemical reaction nor the amount of free energy that is released (Lab Manual 3 pg
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bacteria. Learning how to gram stain‚ use specific media such as MacConkey agar‚ and test antibiotics to see which antibiotic would react best against a specific organism. All these techniques helped me prepare for the final lab‚ identification of an unknown bacterium. For the final lab‚ I received the organism “6A”. To start identifying this organism‚ I did a gram-stain to identify if the organism is gram positive or negative. I created two slides to ensure that I did the stain correctly with similar
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Name Lab Report 1 – June 10‚ 2014 Lab # 4: Melting Point lab Partner: Instructor: The Testing of the Melting Points of p-dichlorobenzene and naphthalene Introduction: Melting point temperature is a physical property of pure substances. It is an intensive property‚ which means the amount of material tested is irrelevant. This lab will determine the melting point temperatures of two known pure substances‚ naphthalene and p-dichlorobenzene‚ using micro-sized quantities and a capillary
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the pi-bonding molecular orbitals: 1 = + 2 2 = + 1.41 (two states) 3 = (two states) 4 = - 1.41 (two states) 5 = - 2 Give the electron configuration for the pi-bonding for the ground state of C8H8. c) What is value for S (total spin quantum number) for the ground state of C8H8. d) What is E‚ the pi-bonding energy‚ for the ground state of C8H8. e) Ed‚ the dlocalization energy‚ is defined as Ed = E - N ( + ) where E is the pi-bonding energy‚ N is the number
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Microbiology 215 exam #2 chap 7‚ 11‚ 12 81-90 essay: 10 points Describe the Kirby Bauer Test? Make sure you describe all the key elements. 1. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis 2. Place antibiotic disks evenly spaced on the inoculated agar plates and incubate at 37C for 24-48 hours. 3. Using sterile technique‚ inoculate 3 nutrient agar plates individually with: a. E. coli b. S. aureus c. M. smegmatis
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Theodor Wilhelm Engelmann was a German microbiologist whose 1882 experiment measured the effects of different colors of light on photosynthetic activity and showed that the conversion of light energy to chemical energy took place in the chloroplast. In 1881‚ he observed the movement of bacteria towards the chloroplasts in a strand of “Spirogyra” algae. Engelmann hypothesized that the bacteria were moving in response to oxygen generated by the photo synthetically active chloroplasts in the algae.
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The Effectiveness of Antimicrobial Agents Bacteria: Clearasil and Proactive Veronica Hillgren 11/16/12 FRI AM Question/ Hypothesis The purpose of my experiment is to find and compare the efficiency of Proactive™ and Clearasil™ acne products on certain bacteria. For myself‚ Clearasil™ works better than Proactive™ does when exposed to my pores‚ which are infested with bacteria. However‚ Proactive™ is widely known and has a large amount
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experiment is to observe how well organisms grow on three different media types such as chemical defined‚ complex‚ selective‚ and differential media. Experimental Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant‚ write our initials‚ and
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