performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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Unknown 2 Scheme “ Fair game” ions Cations: Na+‚ K+‚ NH4+ ‚ Ca2+‚ Mg(OH2)62+ ‚ Al(OH2)63+ Anions: SO42-‚ HSO4-‚ NO3-‚ OH-‚ Cl-‚ CO32-‚ HCO3- Insoluble Compounds: Ca(OH)2‚ CaSO4•2H2O‚ CaCO3‚ MgCO3‚ Mg(OH)2 I. Describe Sample a. Quick description of sample. Ex: Phase‚ color‚ odor‚ crystalline‚ amorphous‚ gel-like‚ powdery‚ etc. II. Flame Test a. Part 1 i. Orange Flame: Na+ is present. K+ ‚ NH4+ ‚ Ca2+‚ Mg(OH2)62 ‚ Al(OH2)63+ may also be present. ii. Purple Flame: K+ is present (no Na+
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The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
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as are their abilities to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding
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Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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Determining pH Using a Red Cabbage Farnoosh Mazarei Food Microbiology April 22‚ 2011 Purpose: To test the pH level of many common household object by use of the juice from a red cabbage. Background: pH is the measure of the acidity or basicity of a solution. The pH scale ranges from 1 to 14 with 1 through 6 being acidic and 8 through 14 being basic; 7 is considered neutral. The use of the red cabbage‚ or red cabbage juice is because red cabbage can be used as an acid/base indicator
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_____________________________________________ Project 3: Identifying the Unknown White Compound by Testing Physical and Chemical Properties _____________________________________________ Michigan State University Chemistry Lab 161 Section 27 19 October 2017 Friday 11:00-1:50 Introduction For this experiment‚ there was an unknown white compound that had to be identified. By testing this compound for different properties it made making an educated guess easier to determine
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