very useful in knowing its risk of toxicity to humans or animals‚ its resistance or susceptibility to antibiotics‚ and determining how to control its growth or kill it altogether. The purpose of these procedures is to discovery the identity of an unknown microbe by observing its reactions to a barrage of chemical and physical tests. Different microorganisms react in different ways‚ due to their function‚ digestibility‚ morphology‚ chemical make-up and other details. By observing the responses to these
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how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms‚ more specifically‚ our unknown bacteria. There are many ways to distinguish and classify bacteria. You can distinguish bacteria from other microorganisms by its size. Bacteria are around one micrometer and yeast is 4-40 micrometer
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Funke‚ Christine L. Case (2010). Microbiology: an introduction (10th ed.). San Francisco‚ CA: Pearson/ Benjamin Cummings. James G. Cappucino‚ Natalie Sherman (2011). Microbiology: a laboratory manual (9th ed.). San Francisco: Benjamin Cummings. Micrococcus luteus. (n.d.). Retrived Jun 11‚ 2012 from the Wikipedia: the free encyclopedia: http://en.wikipedia.org/wiki/Micrococcus_luteus. ----------------------- Sediment Confluent growth Single colony
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PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful for culturing organisms which prefer reduced oxygen levels and increased carbon dioxide levels. The candle jar is not an oxygen free system. This is the best method of culturing a microaerophile. An obligate
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Materials: 1 clean microscopic slide‚ 3% H2O2 solution‚ swabs. Micrococcus luteus‚ Enterococcus faecalis‚ patient G Procedure: 1) Scrape some cells off from each bateria to the slant and place them on glass slide. 2) Place one or two drops of H2O2. Watch for bubbling as an indication of O2 production. 3) Discard the used slide container. Results: Organisms Bubbles formation Catalase Patient G Bubbles Positive Micrococcus luteus Bubbles/O2 formed Positive Enterococcus faecalis No bubbles/No
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provide results. Both the unknown isolate and members of the Micrococcus genus were shown to be obligate aerobes. By using staining methods‚ this proved that the organism is gram positive. Morphology‚ such as‚ orange pigmentation and coccus shape provide similarities to the Micrococcus genus. Physiological tests were shown to be obligate aerobe‚ mesophile‚ neutrophile‚ and osmotolerant organism. Biochemical reactions such as producing enzymes and fermentation are limited. Micrococcus bacteria are found
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The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic‚ measuring 1 micron in length. However‚ colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them. The first two types of bacteria are archaebacteria and eubacteria. Both groups have common ancestors dating to more
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Coccus is a term used to describe a type of morphology that helps us distinguish different kinds of bacteria. The cell’s morphology is only one of the many ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium
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