Staphylococcus epidermidis is one of the most prevalent microorganisms found on the human skin and in the mucous membranes‚ however‚ it is a typically overlooked bacterium because there is very little that is known about it. Though it is not as aggressive as its cousin Staphylococcus aureus‚ Staphylococcus epidermidis is the most frequent cause for nosocomial pathogens‚ especially among newborns‚ the elderly‚ and anyone who has a compromised immune system. These infections are usually associated
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests‚ Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read
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1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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Staphylococcus Aureus (Staph Aureus) is a gram-positive coccal bacterium and is a member of the Firmicutes phylum. It is most commonly found in the respiratory tract or in skin infections and can sometimes occur from food poisoning. This bacterium is very common and can be found in almost all parts of the world. Every year around 500‚000 people in the USA go to the hospital to get treated (National Institutes of Health‚ 1999). Staph Aureus has genetically developed to resist many antibiotics (Larsen
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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Unknown Lab Report Dr. Nathan Cahoone Microbiology 204 December 9‚ 2010 Introduction There are many reasons for knowing the identity of microorganisms. The study and test was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium which I was using unknown #25. Results Unknown #25 had the following morphology on a streak plate: medium sized butyrous cream colored colony. Gram-staining was utilized
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Unknown Lab Report #1 Unknown #1 April 25‚ 2012 Microbiology Spring 2012 MCB2010C Unknown #1 Introduction Identity of a microorganism has proven to be very significant. Doing so can help identify diseases and created treatment and cures for such diseases. As a result‚ various laboratory tests were performed to an unknown microbe (Unknown #1) found in the water of a nearby pond. By identify the microbe‚ the safety of the water will be known to those around it. Materials and Methods
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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