of biological system is usually preferred as the pH system‚ which determines the pH level of dilute aqueous solutions. In this laboratory‚ the data collected from the experiment will be used to graph the titration curves which help to identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since
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The Urea test was used to test both Unknown Gram-positive and Unknown Gram-negative bacteria. One colony of bacteria was used from the Unknown 13A plate and Unknown 13B plate in order to inoculate the Urea Slant. After the slant was inoculated‚ the cultures were placed in an incubator at 35ºC for Gram-positive bacteria‚ and 37ºC for Gram-negative bacteria for a total of 48 hours. If the Urea test were positive‚ the color of the slant would have changed to a hot pink color‚ which indicated the bacteria’s
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Maura Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of
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Determining an Unknown Through Deferential Stains and Biochemical Tests Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification
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Classification for Functional Groups Alcohols Lucas test (Differentiates primary‚ secondary‚ and tertiary alcohols) Reagent: ZnCl2 in conc. HCl Observation: Rate of reaction (tertiary alcohol> secondary alcohol> primary alcohol) Procedure: 2mL Lucas Reagent in test tube+ 3-4 drops of alcohol‚ stopper‚ shake vigorously‚ NOTE time required (less than 10m mins only) to form an emulsion or separate layers. Oxidation (Confirms if alcohol is oxidizable: presence of H in C-OH bond) Reagent:
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Using a Dumas method‚ you are asked to identify the unknown pure liquid by comparing the molar masses of the given possible substances (ethanol‚ methanol‚ isopropanol‚ acetone). Introduction Dumas Method allows us to measure the molar mass of the substance‚ using the Ideal Gas Law. The Ideal Gas Law‚ PV=nRT‚ explains the behaviour of the gases that are near 100kPa and in the room temperature. This equation shows the product of the pressure(P) and volume(V) equals to the product of the number of moles(n)
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Actinomycetes from SedimentMethicillin resistant Staphylococcus aureus (MRSA) frequently causes nosocomial infections‚ is often resistant to most of the antibiotics and is one of the greatest challenges for modern antimicrobial therapy‚ particularly since the emergence of Staphylococcus aureus (S. aureus) with intermediate susceptibility to glycopeptides. (Blanc et al.) In the Philippines‚ it has reported on the prevalence of oxacillin-resistance of S. aureus strains as 18%‚ 24%‚ 18%‚ and 18% in the years
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to cocoa bottom and an unknown girl notes to help on coursework. An unknown girl A traditional indian market scene‚ set in the evening‚ it was to hot to have markets in the day‚ lit up with neon lights She tries to capture the moment‚ she is getting involved in the culture by having her hand hennad. It is a darker shade of your own skin‚ suttle. She is watching this girl ‘icing my hand’(verb(icing)) ‘in the evening bazaar for a few rupees’ india money Unknown girl refers to the lady
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Tuan Tai 1 Duong Tran Tuan Tai Professor Pham Quoc Loc ENGL - 1302 17 September 2014 Some nice quotes from unknown authors In my view‚ everyone love reading always notes their favorite quotes for their life’s guideline. To me‚ I am not really love reading but I love collecting the nice quotes. The quotes bring many meaningful‚ some are evident but some are vague. In addition‚ I also like to collect quotes from the Internet because I do not need to read whole a book and some of them never appear
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determined that Unknown A‚ B‚ C‚ and D are to be identified as the Sprite‚ burger‚ fries‚ and bun‚ respectively. Each Unknown provided evidence that we eventually used to match to each element of the meal. Along with the lab data‚ our prior knowledge also led us to our results. We were able to determine that Unknown A is the Sprite due to the fact that every test was negative except for simple sugars‚ a macromolecule that the majority of sodas contain. We determined that Unknown C were the
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