experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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A Comparative Essay between “Disabled” and “An Unknown Girl” Moniza Alvi reflects on India as her hand is hennaed by “an unknown girl” “in the evening bazaar”. The predominantly upbeat mood of this poem contrasts with the pessimistic mood in “Disabled” written by Wilfred Owen‚ a soldier in the First World War‚ stating his anti-war view through a poem on the life of a young soldier “sewn short at elbow”‚ crippled by war. In his poem‚ the difference between past and present allows Owen to reveal
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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Submitted: October 00‚ 2014 Experiment No. 7 Unknown: 2 Compound: Table 6.1Physical Examination State Liquid Odor Smells like adhesive paste Color Pink-orange Flammability Flammable Table 6.2Physical Constant: Melting Point Parameter Experimental Theoretical (From Literature) Melting Point (oC) Trial 1 Trial 2 Average 88 88 86 86 Table 6.3Solubility Test Sample Solubility Solubility Class Water Ether 5% NaOH5% Na2CO3 5% HClConc. H2SO4 Unknown Sample + + + + + + Basic Compound Table 6
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Purpose: The purpose of this experiment was to be able to apply our knowledge of chemistry to identify 10 unknown elements that were presented to us. We were allowed to use Bunsen burners‚ hydrochloric acid‚ use PH paper to test if the unknown solution is an acid or a base‚ and precipitate each unknown solution with each other. Procedure / Data / Conclusions: Unknown #1: I found solution 1 to be KI. While doing the precipitate reactions‚ I found that it precipitates only
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Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held in the flame
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Chemistry Lab Report Purpose The purpose of this lab is to determine the identity of an unknown liquid by measuring its density and its boiling point and try to match it with those solutions given in Table 2 of experiment 2. Procedure Part A In Part A‚ The main purpose was to find the determination of the density of the unknown (j41) and by doing that we had to determine volumes of the unknown liquid (j41) using three different volumetric devices which are graduated cylinder‚ pipette
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pictures of courtship wherein the patriarchy is still present‚ and an example of this patriarchal view can be seen in N.V.M Gonzales’ short story‚ where the courtship is evident between the male protagonist and Aida. In N.V.M. Gonzales’ “The Bread of Salt”‚ the male protagonist and the way he views his own masculinity and Aida’s femininity‚ as also seen in the story’s literary details and setting‚ reflect a patriarchal picture of courtship where the female is chased and objectified. An analysis of the
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Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
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