Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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Identification of Staphylococcus aureus and epidermidis Wendy Heck Bio 175: General Microbiology Fall 2012-11-21 Staphylococcus aureus is the most pathogenic for humans and Staphylococcus epidermidis is part of the normal flora and is of low pathogenicity. Staphylococcus epidermidis and Staphylococcus aureus are two medically important species of bacteria. A culture from the nose and throat was taken to perform whether or not Staphylococcus epidermidis or Staphylococcus aureus were detected
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Maura Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called a nosocomial infection. S. aureus has a large
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thermotolerant coliforms‚ E. coli)‚ Pseudomonas aeruginosa‚ Staphylococcus aureus and Legionella spp. HPC‚ thermotolerant coliforms and E. coli are indicators in the strict sense of the definition. Non-faecally-derived Can used to determine non-faecal shedding. The absence of these organisms‚ however‚ does not guarantee safety‚ as some pathogens are more resistant to treatment than the indicators‚ no perfect indicator organisms 3. Staphylococcus aureus is a Gram-positive coccal bacterium that is a member
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------------------------------------------------- ------------------------------------------------- EVALUATION OF EFFECTIVITY OF SOAPS AND DETEGENTS AGAINST BACTERIA BACTARIUM EVALUATION
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the principals of living microbes and identifying my unknown bacteria through numerous biochemical and metabolism tests‚ with the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus‚ Staphylococcus aureus‚ Staphylococcus epidermidis‚ Alcaligenes faecalis‚ Escherichia coli‚ and Proteus
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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color from the primary stain is called gram-positive. Bacteria stain differently because of chemical and physical differences in the cell wall. Procedure For this lab we used three cultures; Escherichia Coli‚ Bacillus Subtilis‚ and Staphylococcus Epidermidis. Using a different slide for each sample we started by preparing and fixing each slide. Next we covered the slide with crystal violet for 30 seconds then rinsed with water. Next we covered the slide with Grams Iodine for 10 seconds and rinsed
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