microorganism analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately 0.5 to 1.5 micrometers in diameter‚ it is a facultative anaerobe but grows best in aerobic conditions (Bukhari‚ 2004). Typical hosts of this species are humans and other warm-blooded animals‚ where a favorable temperature of 37℃ is usually maintained in the intestinal tract. S. epidermidis may cause urinary
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Thus‚ identification of microbes is a process of elimination based on logic and carefully-performed tests to determine the capabilities of the unknown microbe. The pieces of evidence used in the microbiology lab are your observations of the shape‚ morphology‚ Gram reaction‚ and the results from biochemical tests performed using a pure culture of an unknown organism. Results are compared to tables of known results for all bacteria with similar shape‚ morphology and Gram reaction to see where similarities
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Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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Lab Session 8: Selective and Differential Media‚ Unknown ID (Enzyme Based Tests) Oct. 25-31st (To be turned in PRIOR to start of recitation for lab 8) Name: _____ ________ Objective: Analyze microbes from last week. Understand the use of antibiotics on microorganisms. Gain more knowledge about selective media and differential media. Practice use of the catalase test‚ coagulase and the oxidase test. Observe microbial flora of the nose. Significance: Understand the use of Mannitol
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of Knowledge (1999). United States of America‚ Grolier Incorporated. Research Update of Mango and Mango Leaf Extract. (n.d). Retrieved August 11‚2010‚ from http://www.mdidea.com/products/herbextract/mangiferin/ research.html. Todar K.(2008). Staphylococcus. Retrieved December 4‚ 2010 from www.textbookofbacteriology.net Williams‚ Y.(n.d.). How to Make Herbal Ointment Remedies. Retrieved October 9‚ 2010‚ from http://www.unexplainable.net/artman/publish/article_ 7674.shtml
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publication: November 19‚ 2008) ABSTRACT The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian‚ Pshdar‚ Sharbazher‚ Khormal‚ Kanakawa and Sulaimani (city center)] against (Staphylococcus aureus‚ Staphylococcus epidermidis‚ Escherichia coli‚ Pseudomonas aerugenosa‚ Klebsiella pneumoniae‚ Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of
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(catalase-negative organisms will not). This test is good for distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. The test uses rabbit plasma that has been inoculated with a staphylococcal colony. The tube is then incubated at 37 degrees Celsius for 1½ hours. If negative then continue incubation up to 18 hours
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resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar was streaked and incubated at 37 degrees Celsius for 24
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REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and
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Organism Result + or - Appearance/Observations Bacillus subtilis Escherichia coli C. Coagualse: identification of Staphylococcus aureus Note: whether the rabbit plasma has remained liquid or become solid due to clot formation Organism Result + or - Appearance/Observations Staphylococcus aureus Staphylococcous epidermidis D. Urease Acitvity: identification of Proteus spp. Note: the color of the urease slant Organism Result + or - Appearance/Observations
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