for detecting infections. Materials 1) Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15) Procedures First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C‚ BS‚ & SA and
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into the cell to be catabolized. Bacillus species are known to produce the exoenzyme‚ amylase. In our experiment‚ we concluded that Bacillus cereus from soil can hydrolyze starch in order to use these starches as a carbon source and use it. But Staphylococcus cannot. Blood agar is an Enriched Media which have been supplemented with 5% sheep red blood cells. BAP tests the ability of an organism to produce hemolysing enzymes that damage/lyses red blood cells (erythrocytes). The degree of hemolysis
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INTRODUCTION Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature‚ this plant has been described to be useful against skin diseases‚ liver troubles‚ tuberculous glands and its use in the treatment of rheumatism‚ hematemesis‚ pruritus‚ leucoderma‚ and diabetes.[1‚2] Besides‚ it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women
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References: Otto‚ M. (2009). Staphylococcus epidermidis--the ’accidental ’ pathogen. Nature Reviews Microbiology‚ 7(8)‚ 555-567. Frank‚ D. N.‚ Feazel‚ L. M.‚ Bessesen‚ M. T.‚ Price‚ C. S.‚ Janoff‚ E. N.‚ & Pace‚ N. R. (2010). The Human Nasal Microbiota and Staphylococcus aureus Carriage. PLoS ONE‚ 5(5)‚ 1-15. Pommerville‚ Jeffrey. (2011). Alcamo’s Fundamentals of Microbiology. 9th ed. Sudbury:
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19.1 Review the anatomy of the skin and mucus membranes with regards to microbial defenses. Section Reference 1: Section 19.1 The Skin‚ Mucus Membranes‚ and Eyes 2) Which of the following is not considered resident microflora of the skin? a) Staphylococcus b) Corynebacterium c) E. coli d) Streptococcus Answer: c Difficulty: Easy Learning Objective 1: LO 19.1 Review the anatomy of the skin and mucus membranes with regards to microbial defenses. Section Reference 1: Section 19.1 The Skin‚ Mucus
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for the initial 1-2 weeks as there is always a possibility of rupture of the cystic bleb. Topical antibiotics should be continued for a period of 2-3 months and then tapered. Early bleb related endophthalmitis is caused by Streptococcus epidermidis‚ Staphylococcus aureus‚ Propionibacterium acnes and other gram positive organisms. Late onset endophthalmitis is caused by Streptococcus‚ gram negative organisms such as Haemophilusinfluenzae‚ Moraxella‚ Pseudomonas and Serratia The treatment of bleb related
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CHAPTER ONE INTRODUCTION The industrial attachment is an important part of the course structure of the university. This is the time students appreciate the theoretical work done in the lecture halls when seen practically. This attachment introduces the student to the outside world in terms of working experience and also prepares them for the future. The department of applied biology therefore introduced the industrial attachment program in its learning curriculum to give students a chance to combine
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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between 4 and 11 depending on the species. The optimum temperature also varies by species. History Catalase was first noticed in 1811 when Louis Jacques Thénard‚ who discovered H2O2 (hydrogen peroxide)‚ suggested its breakdown is caused by an unknown substance. In 1900‚ Oscar Loew was the first to give it the name catalase‚ and found it in many plants and animals. In 1937 catalase from beef liver was crystallised by James B. Sumner and Alexander Dounce and the molecular weight was worked out
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Double-J (DJ) stenting is the commonest procedure performed in urology. Patients with stent‚ develop stent infections and stent-related symptoms. Use of antibiotics in patients with DJ stent is rampant. No uniform data is there to prove advantage of antibiotic prophylaxis. Colonial way of life of microorganisms‚ complex microbial assemblages are responsible for formation of biofilm. Both gram positive and gram negative bacteria produces biofilm. Biofilm is an important virulence factor and is main
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