Title: Bradford Assay: Creating a Standard Curve and Determining Unknown Protein Concentrations Introduction: In biochemistry the ability to determine the quantity of a certain substance in a solution is extremely important. Often protein concentration within a biological organism is essential in determining the function or condition of that organism. If the protein concentration in an environment is increased or decreased it can have detrimental side effects. If there is a decrease in hemoglobin
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world: “The Industrial Revolution was the transition to new manufacturing processes in the period from about 1760 to sometime between 1820 and 1840.” (Unknown) There was many new developments: “This transition
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to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of the unknown; however‚ the solution used for each method was slightly different. Furthermore‚ the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A. The distance moved by the spot could not solely determine the unknown sample and so‚ the
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An Introduction to Qualitative Analysis Purpose: Tests the ions of Mg2+‚ Ca2+‚ Sr2+‚ and Ba2+‚ and identify them separately. By using these observations‚ identify the unknown ion. Then tests the ions of SO42-‚ CO32-‚ Cl-‚ and I-‚identify them separately‚ and use the observation to identify the unknown. Procedure Part I - Qualitative Analysis of Group 2 Elements Mix 0.02M K2CrO4 with each Mg(NO3)2‚ Ca(NO3)2‚ Sr(NO3)2 and Ba(NO3)2 together. Secondly‚ mix 0.1M (NH4)2C2O4 instead
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Organic Chemistry Experiment - Hydrocarbons Thursday‚ April 12‚ 2007 1:34 PM Purpose: To Identify an unknown Hydrocarbon Procedure: Procedure listed in handout "Organic Chemistry Experiment -- Hydrocarbons" Hazards: Open flame and hydrocarbons are flammable. Equations: 1. . 3. . 4. . 5. . Unknown #: 1B s-6 Hyd-3 (colorless liquid) Data/Observations: Test Bromine addition Alkane Mixture of hexane and dichloromethane was originally clear liquid. After adding 3 drops red color Br2/CH2Cl
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Separation of a Mixture: Unknown # 12-Green Chemistry 221 with Professor Thomas Quale May 2012 Formal Lab Report Abstract An unknown sample‚ # 12-Green‚ was separated into its individual variable components‚ iron‚ ammonium chloride‚ silicon dioxide‚ and sodium chloride. The techniques used to separate the components of unknown # 12-Green‚ magnetism‚ sublimation‚ extraction‚ and filtration‚ were chosen based on the unique properties of each component. Using these separation techniques‚ each substance
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Proteus mirabilis The genus Proteus‚ according to Bergey’s Manual of Determinative Bacteriology‚ is a group of straight rod Gram negative bacteria around 0.4-0.8 μm in diameter and 1-3 μm in length. In the microscopic test done‚ it was seen that the unknown culture contained rods that are on the shorter side‚ thus was identified as bacteria that appear to be coccobacilli. Because of the presence of peritrichous flagella‚ species included this genus are motile and most strains swarm in periodic cycles
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behavior. Solutions of each unknown amino acid are made and the change in pH upon adding small amounts of NaOH aliquots of a strong base are monitored. The amino acids are identified using the information represented by the titration curves. The titration curves include the following information: isoelectric points‚ pKas‚ buffering regions and the structures of the amino acids. The results of the experiment were that the unknown that was labeled A was concluded to be lysine‚ unknown B was figured to be
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and purify the unknown D liquid and solid by using its acidic and basic characteristics in a chemically active extraction then to identify the unknowns by analyzing the physical properties on IR and NMR spectrums. The neutral solid was purified through recrystallization and the melting point range was determined to be 47.5oC to 48.9oC. The basic liquid was purified with simple distillation and the boiling point of 135oC was concluded. Through the analysis of IR spectrum of each unknown the structures
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Yvan Mamboukuate 10/09/2013 BIO 101 Lab Report Biological Molecules Introduction: In this experiment‚ simple chemical tests will be performed to identify the presence of various types of biological molecules. These tests take advantage of the fact that different types of molecules have different functional groups and‚ therefor‚ different chemical properties. Thus‚ they will react to test reagents (also called indicators) differently. Materials: Biuret‚ Sudan III‚ Iodine‚ Benedict‚ water
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