wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about
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CH4 Prokaryotic Cells No nucleus Plasma membrane Selective permeability lets molecules pass. Enzymes for ATP production. Photosynthetic pigment on folding (on chromatophores or thylakoids). Leakage occurs if damaged by alcohols‚ ammonium‚ and polymyxin antibiotics. Phospholipid bilayer‚ peripheral proteins‚ integral protein‚ transmembrane protein‚ sterols‚ glycocalyx Movement across Membranes Simple diffusion: Movement of solute from high concentration to low Facilitative diffusion: Solute
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Review Questions 1. Which type of S. pneumoniae strain is the most virulent‚ encapsulated or unencapsulated? 2. What allows S. pneumoniae to adhere to cell walls‚ phosphorylcholine or polysaccharide? 3. Which infections are most commonly caused by S. pneumoniae‚ arthritis & peritonitis or meningitis & bacteremia? both 4. What can society do to help protect itself from antimicrobial resistance‚ increase or decrease antimicrobial usage? Decrease usage 1. Staphylococcus aureus‚
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The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature‚ pH level‚ and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria
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learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation and recording of data the unknown organism can be found. Materials and Methods The procedure for all tests performed was taken from Leboffe and Pierce’s Microbiology: Laboratory Theory
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Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was
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UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
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What Is Microbiology? Microbiology is the study (logy) of very small (micro) living (bio) things. Microbiology is the study of microorganisms. These "bugs" include: bacteria (that’s the Latin plural for bacterium); viruses (that’s the non-Latin plural for virus - virii sounds weird‚ so I don’t say it); and‚ fungi (that’s the Latin plural for fungus - which by now you have guessed‚ or already knew‚ and may not be all that interested to know‚ anyway). Microbiology is actually made up of several
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Theodor Wilhelm Engelmann was a German microbiologist whose 1882 experiment measured the effects of different colors of light on photosynthetic activity and showed that the conversion of light energy to chemical energy took place in the chloroplast. In 1881‚ he observed the movement of bacteria towards the chloroplasts in a strand of “Spirogyra” algae. Engelmann hypothesized that the bacteria were moving in response to oxygen generated by the photo synthetically active chloroplasts in the algae.
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