DeKroon et al. DIGE-based PTMs Analysis of protein post-translational modifications using DIGE-based proteomics Robert M. DeKroon‚ Jennifer B. Robinette‚ Cristina Osorio‚ Sun Yong Jeong‚ Eric Hamlett‚ Mihaela Mocanu and Oscar Alzate Summary Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples‚ and this information is valuable in making inferences about relative protein activity. However
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Some other indicators of kidney function damage or glomerulonephritis within a urine test are white blood cells‚ which are an indicator of infection‚ increased proteins‚ which may indicate nephron damage‚ and increased blood levels of creatinine or urea. Kidney biopsy is a procedure to remove small pieces of the kidneys tissue for examination; a kidney biopsy is always needed to diagnose
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® keep it simple science Copying is permitted according to the Site Licence Conditions only HSC Biology Topic 1 MAINTAINING A BALANCE What is this topic about? To keep it as simple as possible‚ (K.I.S.S.) this topic involves the study of: 1. ENZYMES & HOMEOSTASIS 2. TEMPERATURE REGULATION 3. INTERNAL TRANSPORT SYSTEMS 4. EXCRETION & WATER BALANCE but first‚ an introduction... Living Things are Made of Cells Homeostasis All living things are composed of microscopic units
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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galactose and glucose. In comparison‚ there are only two types of fatty acids‚ those being‚ unsaturated and saturated. The group that exhibits the greatest structural variability is amino acids Q: How does the molecular structure of urea suit its biological function? A: The urea contains two nitrogen atoms for every carbon atom; it is soluble very in water‚ this provides the body with a device that can easily remove access nitrogen (waste). Q: Describe the appearance and arrangement of amino acids in
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I. INTRODUCTION Background of the Study Most of the Filipinos earn a living through agriculture. Throughout the years‚ a lot of fertilizers were improvised‚ mostly for the comfort of the Filipino farmers. Human urine‚ for example‚ is a well-balanced nitrogen-rich quick-reacting liquid fertilizer. It contains nitrogen‚ potassium and phosphorus and other nutrients depending on the diet. The health risks associated with use of human urine in plant production are generally low‚ that is why it’s an
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details of paste prepared in various concentrations are given in Table1. After the preparation of the thickening paste‚ the scoured fabric was printed with the help of screen using following recipe (Kale‚ 1976). 30 Parts Procion colour 30 Parts Urea 230 Parts Warm Water (120ºF-140ºF) 650 Parts Thickening Agent 60 Parts Sodium Bicarbonate 1000 Parts Note:
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became visible at this end allowing the tip to be snapped off. The snapped of tip was then used to puncture holes in the plastic film on the tube’s side covering the last eight tests (adonitol‚ lactose‚ arabinose‚ sorbitol‚ Voges-Proskauer‚ dulcitol/PA‚ urea‚ and citrate). Both caps were then replaced‚ and the tube was incubated for 24 hours at 37 °C (Hébert and Leid and Shand
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filtered from the blood (not as well of a marker for kidney function as inulin because some is secreted‚ but still a good marker of kidney function) and excreted in urine so for her to have more than 0.6-1.2mg/dL in her blood is not normal. Her blood urea nitrogen (BUN) levels are also very high. They should only be around 7-18 mg/dL. Her serum calcium is also high; it should only range from 8.4-10.2mg/dL. Her sodium levels are low (normally 135-145 mg/dL). Her potassium is also low (should be anywhere
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Make Your Own Colloidal Oatmeal What you’ll need: * Oatmeal * Something to Grind Up Your Oatmeal ------------------------------------------------- Home Remedies for Eczema Scars ------------------------------------------------- Eczema is a kind of chronic skin problem where inflammation on the outer layer of the skin happens. Eczema can happen due to excessive stress‚ allergies‚ dandruff‚ change in weather‚ hormonal changes in the body and so on. Some of the symptoms of eczema are skin
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