multiple cloning site (MCS) which is artificially implanted by adding a polylinker sequence to it. The pUC19 plasmid is also altered by inserting a gene that codes for beta-lactamase which confers resistance to the antibiotic ampicillin (Read and Strachan 2011). The MCS occupies the 5’ end of the gene lacZ (Sherwood‚ Willey and Woolverton 2012). This gene codes for only the alpha-peptide of beta-galactosidase‚ an enzyme used to break down the disaccharide lactose into glucose and galactose (Read and
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MBK – Lab Report Name: ____________________ Section: ___________________ Antibiotic Sensitivity The Kirby-Bauer Test Interpret your results as described below. INTERPRETATION: · Place the metric ruler across the zone of inhibition‚ at the widest diameter‚ and measure from one edge of the zone to the other edge. HOLDING THE DISH UP TO THE LIGHT MIGHT HELP. · The disc diameter will actually be part of that number. · If there is NO zone at all
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Bacterial Genetics Worksheet 1. Fill in: Transformation Transduction Conjugation a. Transformation _ naked DNA is involved b. Transduction ____faulty head stuffing may occur c. Conjugation_____ involves exconjugants d. Transduction____ adsorption and injection of genetic material e. Transformation__ competent cells required f. Conjugation ____ an F plasmid encodes attachment proteins g. Conjugation _____used to construct the E. coli minute map h. Conjugation_____
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Food Contamination Definition Food contamination refers to foods that are spoiled or tainted because they either contain microorganisms‚ such as bacteria or parasites‚ or toxic substances that make them unfit for consumption. Food contamination is a serious issue because it results in foodborne diseases. Hence‚ awareness of potential sources of food contamination is an important component of good nutrition. Food contamination refers to the presence in food of harmful chemicals and microorganisms
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Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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of an improvement that has been made in the medical branch of science is the invention of antibiotics. An antibiotic is a substance produced by one organism that can destroy another. The term literally means “against life.” One of the earliest discovered and widely used antibiotics is Penicillin. Penicillin is collected from the Penicillium mold. Alexander Fleming is known for discovering this antibiotic. In 1901‚ he went to St. Mary Hospital to study medicine. In 1914‚ during World War I‚
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Antibiotic Resistant Bacteria A better understanding of the use of antibiotics would help prevent the reality of antibiotic resistant bacteria evolving to the point of human extinction. Antibiotic resistance is a natural process‚ stronger bacteria survive and multiply. Even though antibiotic resistance is a natural process‚ it happens faster when antibiotics are used irresponsibly. Through use of media‚ personal responsibility and research‚ our species could help deter further antibiotic resistance
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30 February 2014 Antibiotic Resistant Bacteria: What is it‚ how to prevent it and who it affects? In the United States alone‚ about 23‚000 people die from antibiotic resistant infections yearly. It is also one of the top 15 most dangerous illnesses in the country (United States House of Representatives). Antibiotic resistance is when bacteria develops a different response to an antibiotic that is its ancestor bacteria. Slight changes in bacteria enable the antibiotic to work and successfully
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SEA-HLM-414 Distribution: General Step-by-step approach for development and implementation of hospital antibiotic policy and standard treatment guidelines © World Health Organization 2011 All rights reserved. Requests for publications‚ or for permission to reproduce or translate WHO publications‚ whether for sale or for noncommercial distribution‚ can be obtained from Publishing and Sales‚ World Health Organization‚ Regional Office for South-East Asia‚ Indraprastha Estate‚ Mahatma
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Antibiotics attack bacteria by targeting a protein‚ or stopping the creation of a cell wall and or how they metabolize sugar. This method of targeting the specific functions of bacteria have allowed antibiotics like Penicillin to save millions of live throughout in the past few decades. As amazing as antibiotics are they have a really smart enemy. Bacteria are cleaver little single-celled organisms. Bacteria can actually barf up the antibiotics we use to fight them. The genetic information bacteria
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