Human Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed
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Group 9 Experiment 4: Work‚ Power‚ and Energy Laboratory Report Gerome Simeon‚ Michael Tiu‚ Mary Grace Anne Trinidad‚ Darrel Aaron Udasco Department of Math and Physics College of Science‚ UST España‚ Manila‚ Philippines Abstract The Experiment entitled “Work‚ Power and Energy” is about relationship between work and power and the concept of energy. Activity 1 is about power and its relationship with work‚ the computed power outputs of each member of the group for going up are 118W‚ 205W
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Investigation into relationship between height of fall of an object and the radius of the crater formed in sand as a result of the impact. By Ivan Cocklove Design Defining the problem and selecting the variables: I will investigate how the height from which a mass falls onto flat surface of sand affects the radius of the crater formed as a result of the impact. The height from which the ball is dropped is affecting the speed at the impact because the higher the drop‚ the longer the ball will
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Introduction to Measurements & Error Analysis The Uncertainty of Measurements Some numerical statements are exact: Mary has 3 brothers‚ and 2 + 2 = 4. However‚ all measurements have some degree of uncertainty that may come from a variety of sources. The process of evaluating this uncertainty associated with a measurement result is often called uncertainty analysis or error analysis. The complete statement of a measured value should include an estimate of the level of confidence associated
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MYP Inquiry Lab Report Guidelines Your lab report will effectively communicate the results of your investigation. Please read all the instructions before starting your lab report and refer back to them as needed during the writing process. 1. All reports must be typed. Use a 12pt font‚ 1” margins all around‚ and double-space your text. 2. Each student must write an independent lab report. Lab partners are encouraged to discuss the results of lab exercises‚ but each student must write a
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Experiment 112-5 Thin Film Interference Introduction When two waves of equal intensity combine‚ the intensity of the resulting wave can be anywhere between zero and four times the intensity of the individual waves‚ depending upon the phase difference between them. When they are in phase (the maxima coincide with maxima and the minima coincide with minima)‚ maximum intensity results. When they are exactly out of phase (the maxima of one wave coincide with the minima of the other wave)‚ the
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Yehleen Habitan ENGL 255 Someday women will have equal pay as men may have been different feel in the work place if the women more dominant in the work place. If more male workers in the work place were very limited to control over the job. In the work place the gender equality aspects needs to be taken into action for both women and men. For many factors associated with inequality such as education level‚ parental leave‚ and salary. While the country never has women president; to meet the
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1. Photo of vehicle 2. Where is the vehicle assembled? The 2018 Toyota Tundra is a pickup truck manufactured in the United States specifically‚ Toyota’s San Antonio‚ Texas‚ factory 3. How does the aesthetic design reflect the technology of the vehicle? These types of Pickup trucks are generally used by people who have a labor intensive job. So‚ it is important for them that the vehicle can handle the demands of their job and also their own personal demands after work. After a long day on the
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My Physical Fitness Assessment PED 117- Physical Fitness for Law Enforcement Carlos Canizales Professor Gotay April 05‚ 2012 Physical fitness is defined as‚ “The ability to perform moderate to vigorous physical activity on a regular basis without excessive fatigue.”(Donatelle‚ P.296) There are two parts of physical fitness made up of eleven components‚ known as skill and health. The skill part of physical fitness contains six components and the health section contains five components‚ which
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A Live Case Analysis of B-12 Construction & Trading Corporation Master of Business Management Graduate School of Business Holy Angel University Submitted by (Group 3): Catap‚ Rose Ann L. Dela Cruz‚ Odesa D. Lacson‚ Edward P. Puno‚ Carolyn M. Sinamban‚ Norman N. August 18‚ 2012 I. Company Background B-12 Construction & Trading Corporation started
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