PROTEINS Abstract The experiment aimed to use the concept of viscosity to study the effects of different denaturants on 1% albumin extract. An Ostwald viscometer was used to measure the flow time of 5 mL of the blank and native protein. These were then denatured by adding 1 mL of denaturant and had their flow time measured. The flow time from the blank to denatured protein is increasing. The specific viscosity and reduced viscosity‚ ηred‚ were calculated from the data obtained. The best denaturant
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cooling; after which the mixture is poured onto ice. The solid product will then be isolated by filtration and recrystallized from methanol‚ in which it is very soluable. A.) Materials: 1) .6mL of concentrated sulfuric acid 2) .30g of methyl benzoate 3) Mixture of .2mL of concentrated sulfuric acid and .2 mL of concentrated nitric acid 4) Ice 5) .2 mL ice cold methanol B.) Procedure in Brief: 1) In a 10 x 100 mm reaction tube add .30g of methyl benzoate and .6 mL of concentrated sulfuric
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the retest used the most efficient and exact method. In the “Volatile Organics” experiment‚ methanol is interpreted as the “unknown waste.” This is displayed through Figure 2‚ where the retention rate is 1.275 minutes and its peak signal is 1.4 mV. The range for methanol due to its similar voltage and retention time‚ when comparing Figure 2a and 2b. Because these chemical mixtures contain acetone‚ methanol‚ and 2-butanone‚ the simplest way to determine the volatility of these chemicals is through
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Post Lab #4- Column Chromatography Organic Chem 3418-2 March 3‚ 2011 Theoretical Background- The fluorene and fluorenone mixture was separated by first dissolving the mixture in heptane. Since “like dissolves like”‚ fluorene dissolves with the non-polar heptane and the polar fluorenone dissolves in the polar ethyl acetate solvent. This phenomenon was illustrated in class before the experiment‚ when it was pointed out why water will not dissolve fluorene‚ fluorenone‚ or transstilbene
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10 MIXING AND AGITATION gitation is a means whereby mixing of phases can be accomplished and by which mass and heat transfer can be enhanced between phases or with external surfaces. In its most general sense‚ the process of mixing is concerned with all combinations of phases of which the most frequently occurring ones are A 7. gases with gases. 2. gases into liquids: dispersion. 3. gases with granular solids: fluidization‚ pneumatic conveying‚ drying. 4. liquids into gases: spraying and atomization
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VOJNOTEHNIČKI GLASNIK/MILITARY TECHNICAL COURIER‚ 2012.‚ Vol. LX‚ No. 2 IMPORTANCE OF LUBRICANT ANALYSIS Perić R. Sreten‚ University of Defence in Belgrade‚ Military Academy‚ Department of Military Mechanical Engineering‚ Belgrade DOI: 10.2298/vojtehg1202156P FIELD: Mechanical Engineering‚ Engines and Motor Vehicles ARTICLE TYPE: Original Scientific Paper Summary: Monitoring the performance of lubricants in practical application has multiple significance for both the consumer and the lubricant
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Fluid 5) Ideal plastic fluid 1. Ideal Fluid: An Ideal Fluid is a fluid that has no viscosity. It is incompressible in nature. Practically‚ no ideal fluid exists. 2. Real Fluid: Real fluids are compressible in nature. They have some viscosity. Examples: Kerosene‚ Petrol‚ Castor oil 3. Newtonian Fluid: Fluids that obey Newton’s law of viscosity are known as Newtonian Fluids. For a Newtonian fluid‚ viscosity is entirely dependent upon the temperature and pressure of the fluid. Examples: water
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Abrasive flow machining 1. Introduction Abrasive flow machining (AFM) was firstly developed fifty years ago by Extrude Hone Corporation‚ USA. By using the liquid viscoelastic material with easy flowability made of polymer and abrasive grains‚ AFM can be used to polish‚ deburr and radius for the workpiece. There are three kinds of AFM: one-way AFM‚ two-way AFM and orbital AFM [1]. In this case to manufacture the cylinder heads‚ consider of two-way AFM is much more widely used and its technology
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Measure the weight of each individual and place in a 1.5 mL microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next‚ go to the fume hood and add 500 L of chloroform to each tube. Spin the tubes in a centrifuge
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PROJECT REPORT ON MANUFACTURING PROCESS AND APPLICATIONS OF GUAR GUM CARRIED ON AT ADARSH GUAR GUM UDYOG
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