CHEMISTRY 221 LABORATORY�QUANTITATIVE ANALYSIS EXPERIMENT: DETERMINATION OF ASCORBIC ACID IN VITAMIN C TABLETS THEORY: Ascorbic acid‚ C6H8O6‚ MM = 176.13‚ can be oxidized to form dehydroascorbic acid by bromine via the following reaction: The bromine or iodine used in the titration is generated by adding an excess amount of KBr or KI to an acidified solution of the sample according to the following reaction: BrO3- + 5 Br- + 6 H+ 3 Br2 + 3 H2O IO3- + 5 I- + 6 H+
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: THE VITAMIN C CONTENT OF FRUIT JUICES AND TABLETS DATE OF EXPERIMENT : 1ST AUGUST 2013 DATE OF SUBMISSION : 27TH AUGUST 2013 LECTURER : PUAN ZAKIAH BT. ZAKARIA OBJECTIVE * To determine the vitamin C content in fresh fruit juice * To determine the vitamin C content in commercial fruit juice * To determine the vitamin C content in tablet
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Investigating Vitamin C Background Vitamin C is found in green vegetables‚ fruits‚ and potatoes. It is essential for a healthy diet. The chemical name for vitamin C is ascorbic acid. Ascorbic acid is a good reducing agent and therefore it is easily oxidised. Methods for the detection of vitamin C involve titrating it against a solution of an oxidising agent. Where to start There are several oxidising agents that can be used and a commonly used one is 2‚6-dichlorophenol-indophenol or DCPIP. You need
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IB Biology Lab – Vitamin C content of food Aim: To compare the vitamin C content of different fruits using the decolourisation of DCPIP from blue to clear as the indicator. • You first need to estimate the volume of a known concentration of vitamin C solution required to decolourise 2 cm3 of DCPIP solution. • Then you need to determine the volume of fruit juice that decolourises 2 cm3 of DCPIP solution. This gives an estimate of the concentration of vitamin C in fruit juice. • This
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Investigating the vitamin C content of fruits and fruit juice Aim: In this investigation‚ I aim to measure and compare whether there is a higher content of vitamin C in a fruit or a juice by measuring the volume of the sample required to decolourise a solution of dichlorophenolindophenol (DCPIP). Introduction: For this experiment‚ I aim to investigate and compare the content of vitamin C in the fruit against the fruit juice. The theory of this method is a titration with dichlorophenolindophenol (DCPIP). DCPIP
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Measuring the Vitamin C content in a variety of fruit juices Background Information Vitamin C‚ also called ascorbic acid‚ is a water soluble vitamin. It is a powerful reducing agent that is able to decolourise blue DCPIP (2.6-dichlorophenol indophenols). The decolourisation of DCPIP can therefore be used to calculate the vitamin C content of a variety of fruit juices. Research Question – To determine how the content of Vitamin C (%) will differ in fresh fruits (orange‚ mango‚ lemon‚ lime‚ blackcurrant
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Abstract Vitamin C‚ also known as ascorbic acid‚ is a water soluble vitamin that is regarded as one of the safest and most effective nutrients. Vitamin C can be found in most fruits and vegetables. The goal of the experiment is to find out the concentration of vitamin C in three citrus fruits‚ orange‚ lemon and lime‚ and to compare them and find out which fruit contains the highest concentration of Vitamin C. By using the juice from each fruit to make a solution to combine with 10cm³ of DCPIP until
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Determination of Vitamin C by an Iodometric Titration Purpose: The goal of this lab is to determine the concentration of vitamin C in juices and Real Lemon. A redox titration‚ involving an iodometric method‚ will be used to do the analysis. The samples will be classified by their Vitamin C content. Introduction: Although most mammals can synthesize vitamin C‚ or ascorbic acid (C6H8O6)‚ from sugars‚ man must ingest considerable quantities of this substance. The National Academy of Sciences recommends
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Vitamin C also known as ascorbic acid is vital to our body. It can strengthen our body to resist against infection. It is known that deficiency of vitamin C can cause scurvy. Vitamin C is present in various fruits. Since vitamin C is a reducing agent‚ it can be deactivated by a wide range of oxidizing agents including atmospheric oxygen. Therefore‚ precautions are needed to prevent or minimize the degradation of vitamin C during sample preparation. The ascorbic acid exists in two forms which
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Determination of the concentration of vitamin C in fruits/vegetables by using the DCPIP test (2008) Principle : Vitamin C is a strong reducing agent. It can decolorize the blue dye DCPIP. The amount of vitamin C in a food sample can be estimated by the amount of it that is used to decolorize a fixed amount of DCPIP. Apparatus : Test tube‚ clean and dry Test tube rack 5 ml syringe or a good quality squeezing pipette 1 ml pipette
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