the vitamin C content of different fruit juices. To identify which fruit juices has higher vitamin C content and the lowest vitamin C content. To determine the standard curve of vitamin C based on percentage of vitamin C. To differentiate between fresh juices and carton juices. To consider the reliability and validity of the results. PROBLEM STATEMENT Which type of fruit juices has the most vitamin C content? HYPOTHESIS The smaller the amount of the fruit juices to decolourise DCPIP solution
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CHEMISTRY 221 LABORATORY�QUANTITATIVE ANALYSIS EXPERIMENT: DETERMINATION OF ASCORBIC ACID IN VITAMIN C TABLETS THEORY: Ascorbic acid‚ C6H8O6‚ MM = 176.13‚ can be oxidized to form dehydroascorbic acid by bromine via the following reaction: The bromine or iodine used in the titration is generated by adding an excess amount of KBr or KI to an acidified solution of the sample according to the following reaction: BrO3- + 5 Br- + 6 H+ 3 Br2 + 3 H2O IO3- + 5 I- + 6 H+
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Investigating Vitamin C Background Vitamin C is found in green vegetables‚ fruits‚ and potatoes. It is essential for a healthy diet. The chemical name for vitamin C is ascorbic acid. Ascorbic acid is a good reducing agent and therefore it is easily oxidised. Methods for the detection of vitamin C involve titrating it against a solution of an oxidising agent. Where to start There are several oxidising agents that can be used and a commonly used one is 2‚6-dichlorophenol-indophenol or DCPIP. You need
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IB Biology Lab – Vitamin C content of food Aim: To compare the vitamin C content of different fruits using the decolourisation of DCPIP from blue to clear as the indicator. • You first need to estimate the volume of a known concentration of vitamin C solution required to decolourise 2 cm3 of DCPIP solution. • Then you need to determine the volume of fruit juice that decolourises 2 cm3 of DCPIP solution. This gives an estimate of the concentration of vitamin C in fruit juice. • This
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Investigating the vitamin C content of fruits and fruit juice Aim: In this investigation‚ I aim to measure and compare whether there is a higher content of vitamin C in a fruit or a juice by measuring the volume of the sample required to decolourise a solution of dichlorophenolindophenol (DCPIP). Introduction: For this experiment‚ I aim to investigate and compare the content of vitamin C in the fruit against the fruit juice. The theory of this method is a titration with dichlorophenolindophenol (DCPIP). DCPIP
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Determination of the concentration of vitamin C in fruits/vegetables by using the DCPIP test (2008) Principle : Vitamin C is a strong reducing agent. It can decolorize the blue dye DCPIP. The amount of vitamin C in a food sample can be estimated by the amount of it that is used to decolorize a fixed amount of DCPIP. Apparatus : Test tube‚ clean and dry Test tube rack 5 ml syringe or a good quality squeezing pipette 1 ml pipette
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tamin C‚ ascorbic acid‚ is one of the most important vitamins found in citrus juices‚ including orange juice. Often projects find that orange juices made from frozen concentrated orange juice (FCOJ) have the highest vitamin C levels as compared to freshly squeezed or not-from-concentrate (NFC) juices. This is probably due to the fact that vitamin C degrades over time in fresh and NFC‚ but doesn’t degrade as much in FCOJ due to it being frozen until reconstitution. If one is comparing a NFC product
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Measuring the Vitamin C content in a variety of fruit juices Background Information Vitamin C‚ also called ascorbic acid‚ is a water soluble vitamin. It is a powerful reducing agent that is able to decolourise blue DCPIP (2.6-dichlorophenol indophenols). The decolourisation of DCPIP can therefore be used to calculate the vitamin C content of a variety of fruit juices. Research Question – To determine how the content of Vitamin C (%) will differ in fresh fruits (orange‚ mango‚ lemon‚ lime‚ blackcurrant
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Abstract Vitamin C‚ also known as ascorbic acid‚ is a water soluble vitamin that is regarded as one of the safest and most effective nutrients. Vitamin C can be found in most fruits and vegetables. The goal of the experiment is to find out the concentration of vitamin C in three citrus fruits‚ orange‚ lemon and lime‚ and to compare them and find out which fruit contains the highest concentration of Vitamin C. By using the juice from each fruit to make a solution to combine with 10cm³ of DCPIP until
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Determination of Vitamin C by an Iodometric Titration Purpose: The goal of this lab is to determine the concentration of vitamin C in juices and Real Lemon. A redox titration‚ involving an iodometric method‚ will be used to do the analysis. The samples will be classified by their Vitamin C content. Introduction: Although most mammals can synthesize vitamin C‚ or ascorbic acid (C6H8O6)‚ from sugars‚ man must ingest considerable quantities of this substance. The National Academy of Sciences recommends
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