Lab #3: Ion Exchange Chromatography Objective The purpose of this experiment was to separate proteins on the basis of their net charge at a particular pH. In cation exchange chromatography positively charged molecules are attracted to a negatively charged column. Conversely‚ in anion exchange chromatography‚ negatively charged molecules are attracted to a positively charged column. Experimental results could be monitored in a predictable way by controlling running pH‚ salt concentration‚ and by
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Buffer solutions and common ion effect A buffer solution resists (or buffers) a change in its pH. That is‚ we can add a small amount of an acid or base to a buffer solution and the pH will change very little. How to calculate pH of buffer solution containing both acid and conjugate base? Dissociation constant definition 1.1 can be rearranged into or (note that due to sign change [A-] was moved to nominator). This is so called Henderson-Hasselbalch equation (or buffer equation)
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molecules which consists of carbon‚ oxygen‚ hydrogen and nitrogen. All proteins are made up of one or more polymers‚ known as polypeptides. Its monomers are amino acids. Protein can be divided into primary structure‚ secondary structure‚ tertiary structure and quaternary structure. It is important for all living things in the world as they form enzymes‚ antibodies and hormones. Materials and Procedures: Results and Discussions: Test for Carbohydrates Result Starch suspension When the
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production of light as the result of a chemical reaction with little or no heat produced. The glow sticks can operate by causing two chemicals to mix. A common reaction used for these devices is hydrogen peroxide and phenol oxalate ester. The hydrogen peroxide is kept in a thin‚ small glass vial in the middle of the stick‚ and the second chemical phenol oxalate ester is in the main body of the light stick. Bending the light stick in the middle causes the small glass vial of hydrogen
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for Experiment #7 Student’s Name __________Lisa __________ Date of Experiment _08/03/2013__________ Date Report Submitted ______08/05/2013___________ Title: Identification of Metallic Ions Purpose: To perform and observe the flame tests of some alkali and alkaline earth metal ions. Understanding Light Introduction: Electrons from an atom can absorb energy and become excited to move from the stable “ground state” energy level to a higher energy level. Imagine one of
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The Nervous System II: Ion Channels 1. List four neurotransmitters that bind to ion channels‚ these neurotransmitters are called __directly____-acting neurotransmitters. a. ACh b. glutamate c. GABA d. Glycine 2. a. The binding of ACh opens ion channels in the dendrites or cell body that permits both _sodium______ and _potassium____ to move through them. b. Which ion would move into the cell? _sodium____out of the cell? _potassium_____________ c. Which ion has the greatest electrochemical
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all having their own drawbacks. But‚ the one that will be focused upon in this analysis is hydrogen as a source of fuel‚ and the technology leading the way to making it a reality. This technology would affect everyone in one way or another if correctly used. So we must ask if this technology is beneficial to us‚ how it might hurt us‚ and whether it is worth pursuing from an ethical stance. Background: Hydrogen has already been under the micro scope for many years as an alternative fuel source to us
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By simply comparing the reactions that occurred when each metal was added to HCl‚ we are able to rank the reactions. Metals that reacted with HCl to produce hydrogen gas would be considered more active that those that exhibited no reaction. Therefore‚ the metal that reacted the most vigorously in acid would be the most reactive metal. In addition‚ the metal that displayed no reaction when added to all of the
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First recognized as a chemical compound in 1818‚ hydrogen peroxide is a clear‚ colorless liquid most often used for cleaning or disinfecting(Britannica‚ 2013). Since pure hydrogen peroxide is unstable‚ it is found in stores as an aqueous solution. The chemical formula for hydrogen peroxide is H₂O₂ which means there are two hydrogen atoms and two oxygen atoms in the compound(Liebeskind‚ 2013). Seed germination is simply the process in which a seedling sprouts from a seed and begins it’s growth. The
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System I: Ion Channels 1. What structures in the cell membrane function as ion channels? Integral Proteins 2. Ion channels are selective for specific ions. What three characteristics of the ions are important for this selectivity? 1. Charge 2. Size 3. Amount of water the ion attracts 3. Channels can be classified as either gated or nongated channels. A sodium channel that is always open would be classified as a/an __________nongated______________ channel. 4. Would sodium ions move into
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