2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
Premium Plasmid Gene DNA
The digestive tract is a continuous tract throughout the body that interfaces with the environment at both its origin ‚the oral cavities‚ as well as‚ at its termination‚ the anus. It is divided into different segments beginning with the oral cavity and followed by the pharynx‚ esophagus‚ stomach‚ small and large intestine‚ and the anus. There are also accessory digestive organs involved including the salivary glands‚ liver‚ pancreas‚ and gallbladder. Digestion begins in the oral cavity where
Premium Digestion Stomach Small intestine
Digestive System * uniquely constructed to perform its specialized function of turning food into the energy you need to survive and packaging the residue for waste disposal. Parts of the digestive system: 1) MOUTH * is the beginning of the digestive tract; * digestion starts here when taking the first bite of food. * Chewing breaks the food into pieces that are more easily digested‚ while saliva mixes with food to begin the process of breaking it down 2) ESOPHAGUS
Premium Digestion Stomach Small intestine
Digestive System We all have to eat food to survive when the average person eat food we don’t think about all the things your body have to do just to digest what we ate this short essay I will explain to you the process of how we digest food. Digestion times vary between people but on the average it takes 24-72 hours for our body’s to digest food. The first process with digestion is ingestion which means the chewing process while we chew food it’s broken up in the mouth with saliva and forms
Free Digestion Small intestine Digestive system
Nika Braiman 03/11/13 Digestive System Disorders Go to the following website: http://www.nlm.nih.gov/medlineplus/digestivesystem.html Review the structures of the digestive system using the interactive diagram. When you click on each digestive organ‚ a list of disorders related to that structure will be displayed. Complete the chart by matching the disorder with the structure involved with the disorder. Click on the disorder to get the description. Many disorders affect more than one structure
Premium Cancer Digestion Gastroenterology
LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
Premium Enzyme PH Blood
DIGESTIVE SYSTEM FUNCTION -The digestive system is a group of organs working together to convert food into energy and basic nutrients to feed the entire body. Food passes through a long tube inside the body known as the alimentary canal or the gastrointestinal tract (GI tract). The alimentary canal is made up of the oral cavity‚ pharynx‚ esophagus‚ stomach‚ small intestines‚ and large intestines. In addition to the alimentary canal‚ there are several important accessory organs that help your body
Premium Digestion Gastroenterology Digestive system
temperature on the enzyme activity was that the reaction’s rate would increase as the temperature increased‚ until they go over the optimum temperature where the enzymes denature and the reaction’s rate quickly drops to zero. At 5 degree C the rate is 0.00059mole PNP/min. This then increases to 0.01031mmoles PNP/min at a temperature of 50 degree C. The rate then drops drastically to -0.00215moles PNP/min. This point is where the enzymes have been denatured and have no activity‚ shown as the last point
Premium Enzyme Chemical reaction Catalysis
ASSAYING PLANT SUBSTANCES FOR ANTIBACTERIAL ACTIVITY PURPOSE To test plant extracts to see if they possess antibacterial activity. PROCEDURE First create a list of plant extracts being tested and choose 2 extracts per group. The plants being tested are Oregano‚ Tea tree‚ Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of
Premium Bacteria Microbiology Escherichia coli
REVEREND HENRY WARD A PIONEER OF EARLY CHILDHOOD EDUCATION Rev Henry Ward served as a United Church Minister in Jamaica. Seeing the need for childcare for working mother’s he established the first play center in 1938 that served children under six years old. Soon after he began a campaign to formalize education and play at these centers along with his colleagues. Surprisingly‚ he was met with resistance by parents who disliked the term play center. The term "play center" was changed to Basic
Free Teacher Education School