CHEM 3152-003 Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C
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soaring. As we hug and run over to the bleachers‚ I recall my childhood romanticisms of spaceships and faraway planets‚ and how it has motivated me to explore more in the disciplines of science and technology than I had ever imagined. While these fields initially interested me simply due to star-struck imagination‚ through my involvement in the Bartlett High School Academy‚ VEX Robotics‚ and other similar clubs I have found a love for engineering and technology. Yet‚ my aspirations still exist of
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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useful solvent for many chemical processes. The solid was already in powder form therefore we did not have to crush it but it the unknown was in a tablet form‚ one would need to reduce it to a powdery-like form in order to perform this Thin-Layer Chromatography experiment. The powder solution was thoroughly mixed with a glass rod in the test tube containing
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Comparison and Contrast of Division 13 and Division 14 PSY/700 December 12‚ 2011 Tara Washington University of Phoenix Comparison and Contrast of Division 13 and Division 14 Industrial and organizational psychology is represented by two divisions: 1) Division 13 – Society of Consulting Psychology (SCP); and Division 14 – Society of Industrial and Organizational Psychology (SIOP). The following paragraphs comprise of the history‚ mission‚ community involvement‚ and resources
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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profits and community acceptance. Home Depot cannot afford another failure in Argentina or South America. (Home Depot. 2013). Home Depot: Home Depot is the 4th largest retailer in the US‚ the 5th largest retailer in the world and ultimately the world ’s largest home improvement specialty retailer. In 35 years‚ Home Depot operates 2‚257 retail stores in 50 states‚ the District of Columbia‚ Puerto Rico‚ U.S. Virgin Islands‚ Guam‚ Canada‚ and Mexico. The foreign locations (294) generate just 12% of Home
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Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens‚ using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water‚ D. Sellers used
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