Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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and the optical path length‚ b. ABS = log (Io/I) = εbC or abC at any one wavelength ε = molar absorptivity (L/mole-cm) a = absorptivity (L/g-cm) b = path length (cm) C = concentration (moles/L or g/L) (depends on whether you are using ε or a to represent absorptivity) The absorbance of a solution containing more than one chromophore will be equal to the sum of the absorbances of each of the components at any one wavelength. ABStotal = ABS1 + ABS2 + ... ABS n where the subscripts refer to the
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further studies of wavelengths and the spectrum. When a metal in a compound reacts in a flame and produces a color‚ it helps to show the strongest color in the emission spectrum. The purpose of performing these operations on the flame was to see how different metals would react. Using a spectroscope‚ it is possible to observe the line emission spectrum produced by sunlight‚ artificial light‚ and various other gases. The operations performed relates to the bands of light and wavelength studied in class
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of fuel and oxidant. The sample is vaporized in to atoms and these atoms absorb the light from the Hollow cathode lamp. The wavelengths are adjusted to particular elements which we analyze from the complex sample‚ so there is no interference with measurement and this gives us both sensitivity and selectivity of elements. To reduce the back ground interference the wavelength of interest is isolated by the monochromator placed between the sample and detector. Now let us go in to detail about Flames
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laser has different properties‚ such as wavelength‚ that allow the laser energy to be absorbed by different compounds. A laser that is well absorbed by water would be used on blanched tissue‚ and a laser that is well absorbed by skin pigments would be used on skin or gums. The argon laser is most commonly used on skin tissue because it well absorbed by melanin and hemoglobin. The argon laser operates at two wavelengths of 488nm and 514nm. The 488nm wavelength is used to catalyze dental resin polymerization
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INTRODUCTION: The electromagnetic spectrum is the range of all possible frequencies of electromagnetic radiation. Radiation can be said to be energy that travels and spread as it goes. The "electromagnetic spectrum" of an object has a different meaning‚ and is instead the characteristic distribution of electromagnetic radiation emitted or absorbed by that particular object. We tend to witness different radiations of the electromagnetic spectrum without knowing‚ example is the visible light that
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species has been excited and returns to its ground electrical state. Two spectra are produced: emission and excitation. Excitation is similar to absorption‚ where emission records the intensity of the radiation emitted by the species as it relates to wavelength. Fluorescence is highly sensitive method and can be used in many industries‚ including food analysis‚ pharmacy‚ and natural products. In order to fluoresce‚ molecules must be rigid‚ and unable to rotate so that the energy cannot be dissipated
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1. Based on your observations in Experiments 1 and 2‚ prepare a single table listing an approximate band gap energy‚ Eg compared to composition of the LEDs‚ as well as the voltage across each LED. Recall that Eg = hc/λ. Composition Color Wavelength λ Band Gap Eg Voltage GaP0.40As0.60 Red 653.6 nm 0.0304*10-17J 1.394 V GaP0.65As0.35 Orange 625.3 nm 0.0318*10-17J 1.544 V GaP0.85As0.15 Yellow 594.1 nm 0.0335*10-17J 1.573 V GaP1.00As0.00 Green 563.2 nm 0.0353*10-17J
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UNIT 1 Modern Physics 1.1 CLASSICAL PHYSICS Newtons laws of motion are the basis of the most elementary principles of classical physics. Equations based on these laws are the simplest and they are suitable for solution of simple dynamical problems‚ such as the motion of macroscopic bodies‚ Lagranges equations‚ Hamiltons equations and Hamiltons principle are also fundamental principles of classical mechanics‚ because they are consistent with each other and with Newtons laws of
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Benjamin Kleveland 502 October 26‚ 2014 Lab 7 Report Lights Distance and Wavelength Effect on Photosynthesis Photosynthesis and cellular respiration are often mistaken as the same thing. Although they are similar in many ways‚ photosynthesis and cellular respiration are the exact opposite of each other. Not figuratively‚ but literally the reverse (Photosynthesis). They incorporate the others products while adding some outside energy to create a never ending cycle. This brings us to the photochemical
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