WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
Premium Western blot Molecular biology Gel electrophoresis
The Western Ghats or the Sahyādri constitute a mountain range along the western side of India. It is a UNESCO World Heritage Site and is one of the eight hottest hotspots of biological diversity in the world. It is sometimes called the Great Escarpment of India. The range runs north to south along the western edge of the Deccan Plateau‚ and separates the plateau from a narrow coastal plain along the Arabian Sea. The range starts near the border of Gujarat and Maharashtra‚ south of the Tapti river
Premium Western Ghats
there a heaven and hell? Eastern and Western philosophy are similar but different in many ways. Eastern philosophy includes the various philosophies of ancient China and India‚ but can also include Islamic‚ Jewish‚ and Persian philosophies. Western philosophy refers to the philosophies developed by the ancient Greeks and Indians. There are many differences between Eastern and Western philosophy. One is the east uses concepts by intuition while the west uses
Premium Philosophy
did you prepare for the Midterm Proctored Exam? (3.33pts) - By preparing for my Midterm Proctored Exam‚ I would study my notes daily. Every day I would try to do a quiz after I study a chapter or two chapters to boost my knowledge. I would also‚ print off some extra notes and diagrams off the internet or from the PowerPoints that thought would be useful for me to memorize everything that I needed to know. During the three days before the exam‚ I started to go by the midterm study guide and highlighted
Premium Education Learning High school
Name: Tutor/ professor: Institution: The western tradition Introduction Renaissance is a French term meaning rebirth is a perfect description of the economic as well as intellectual changes that happened in Europe continent from fourteenth century through the sixteenth century. In the course of the renaissance era‚ the whole of Europe sprung from stagnation in terms of economic status which was dominant in the middle ages to a period of economic or financial prominence. Of much greater importance
Premium Renaissance Protestant Reformation Middle Ages
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
Premium Molecular biology Protein Gel electrophoresis
antiquity that exists in the Eastern cultures. We think 300 years is a long time when in Europe traditions can be traced back a couple thousand years and in India they can trace Hinduism and their way of life back 4‚000 years. The very nature of western religion is nothing but change. It has its roots in Greek and Roman polytheism and to go from polytheistic to monotheistic is a huge‚ 180 degree turn and about the biggest change
Premium Religion Hinduism Islam
first early humans and how they went from underdeveloped civilizations or communities to becoming the first early civilizations. While in the second chapter a new age begins as the Bronze Age begins to transition into the Iron Age as the Middle Eastern culture and values became widely shared Amongst the Egyptians and the Mesopotamians which was known as the cosmopolitan era. Which meant that lifestyle and cultures were shared in these civilizations where people such as the Amorites‚ Kassie’s‚ and the
Premium Roman Empire Ancient Rome Ancient Greece
Politics and the State in Western Europe ca 1450-1521 Due to the ineffective leadership‚ the nation states of Frances‚ England and Spain utilized aggressive methods to rebuild their governments in the fifteenth century. This was accomplished though the revival of the monarchy and the unification of nations. The French leaders led their people and nation states to success. Charles VII‚ a sovereign viewed as weak and frail‚ proved himself a powerful leader. Charles (reigned from
Premium Jews Henry VIII of England Nation
Sakija Wilkinson HST 140 WA/4 WA2 Geography Influenced Civilization Geography influenced civilization that arose in china‚ in so many ways. One way was the yellow river in which ancient china basically grew out of. All of the earliest civilizations arose on flood plains of major rivers because these lands provided the fertile land required to support their civilization. The yellow river was used to contribute to china’s civilization by also being as a highway to unify and control. There was also
Premium China Warring States Period Qin Shi Huang