WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
Premium Western blot Molecular biology Gel electrophoresis
The Western Genre Many movies come out and pertain to a certain genre. The western genre is an example of that. But then there raises a question‚ what exactly makes this movie a western film? There may also be the question‚ what can the viewer expect to see in a western film? We’ll to start by answering the first question. Western films all have similar plot lines‚ objects‚ and elements within the genre that will be extend to most if not all other western movies. For example‚ true westerns typically
Premium Native Americans in the United States United States Cowboy
Categories » Education and Communications » Research and Review » Essays How to Write a DBQ Essay Edited by Teresa‚ Lillian May‚ Maluniu‚ Chris Hadley and 21 others 55 Article Edit Discuss You’re in an AP History class and you have a DBQ essay coming up. Don’t panic! As long as you’ve been (mostly) paying attention in class and read these steps‚ you’ll do just fine. EditSteps Prewriting for your DBQ Essay 1Know that you will be given 15 minutes to read the prompt‚ analyze the documents
Premium Writing Essay Thought
The Western Ghats or the Sahyādri constitute a mountain range along the western side of India. It is a UNESCO World Heritage Site and is one of the eight hottest hotspots of biological diversity in the world. It is sometimes called the Great Escarpment of India. The range runs north to south along the western edge of the Deccan Plateau‚ and separates the plateau from a narrow coastal plain along the Arabian Sea. The range starts near the border of Gujarat and Maharashtra‚ south of the Tapti river
Premium Western Ghats
there a heaven and hell? Eastern and Western philosophy are similar but different in many ways. Eastern philosophy includes the various philosophies of ancient China and India‚ but can also include Islamic‚ Jewish‚ and Persian philosophies. Western philosophy refers to the philosophies developed by the ancient Greeks and Indians. There are many differences between Eastern and Western philosophy. One is the east uses concepts by intuition while the west uses
Premium Philosophy
Name: Tutor/ professor: Institution: The western tradition Introduction Renaissance is a French term meaning rebirth is a perfect description of the economic as well as intellectual changes that happened in Europe continent from fourteenth century through the sixteenth century. In the course of the renaissance era‚ the whole of Europe sprung from stagnation in terms of economic status which was dominant in the middle ages to a period of economic or financial prominence. Of much greater importance
Premium Renaissance Protestant Reformation Middle Ages
Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
Premium Molecular biology Protein Gel electrophoresis
antiquity that exists in the Eastern cultures. We think 300 years is a long time when in Europe traditions can be traced back a couple thousand years and in India they can trace Hinduism and their way of life back 4‚000 years. The very nature of western religion is nothing but change. It has its roots in Greek and Roman polytheism and to go from polytheistic to monotheistic is a huge‚ 180 degree turn and about the biggest change
Premium Religion Hinduism Islam
Sean Vasquez DBQ The period 1750 to 1776 is often referred to as the “Road to Revolution” due to the in increased tension between the colonists and England. The English parliament passed numerous acts that increased colonial taxes‚ angering the colonists. Between 1750 and 1776‚ in response to the tax laws‚ the colonies united and formed the ultimate identity of the United States of America. As early as 1754‚ the colonial unity was beginning to be discussed by several colonies and individuals
Free American Revolution United States Thirteen Colonies
Politics and the State in Western Europe ca 1450-1521 Due to the ineffective leadership‚ the nation states of Frances‚ England and Spain utilized aggressive methods to rebuild their governments in the fifteenth century. This was accomplished though the revival of the monarchy and the unification of nations. The French leaders led their people and nation states to success. Charles VII‚ a sovereign viewed as weak and frail‚ proved himself a powerful leader. Charles (reigned from
Premium Jews Henry VIII of England Nation