Solve the Murder Koreen Clarke October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette
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Procedure: Day 1: Buffer preparation First‚ the buffer was prepared by using the formula as follows: Figure 1: Calculation for prepare 0.5 M Tris buffer at pH 6.8 3.033 g of Tris was weighed and placed in 400 mL beaker. Then‚ 25 mL of distilled water was added into the beaker that contained Tris. The mixture was dissolved using the stirring rod‚ and then the magnetic stirring bar was placed in the beaker for further dissolve when measuring the pH. The pH meter was used to measure the solution
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com/home/world-news/article/16200962‚ economic-social-beneits.aspx‚ 2012 Oter‚ Z Sorupia‚ E. (2005). Proceedings of the Eastern Asia Society for Transportation Studies‚ Vol Timur‚ A. and Olali‚ H. (1988). Tourism Economy‚ Ankara‚ Ankara Publication Westlake J May 24‚ 2012 – May 26‚ 2012
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IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING‚ VOL. 7‚ NO. 2‚ FEBRUARY 2014 503 Integration of LiDAR Data and Orthophoto for Automatic Extraction of Parking Lot Structure Lihua Tong‚ Liang Cheng‚ Manchun Li‚ Jiechen Wang‚ and Peijun Du Abstract—To overcome the challenges of parking lot structure extraction using optical remote sensing images‚ this study proposes an automatic method for the extraction of parking lot structure by integrating LiDAR data
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CONTROLLED COPY NO :………………………… CONTENTS 1.0 PURPOSE 2.0 SCOPE 3.0 REFERENCES 4.0 DEFINITIONS 5.0 PROCEDURE 6.0 DOCUMENTATION | | Issued for Use | | | | | | Issued for Comment | | | | REV | DATE | STATUS | BY | REVIEWED | APPROVED | CONTROLLED COPY NO :………………………… CONTENTS 1.0 PURPOSE 2.0 SCOPE 3.0 REFERENCES 4.0 DEFINITIONS 5.0 PROCEDURE
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You never drive under the influence of alcohol and use a designated driver on social occasions. However‚ this does not guaranty that you won’t get involved in an alcohol related accident with another driver who is intoxicated. In spite of law enforcement efforts‚ driving under the influence is still an ongoing problem. The problem is especially acute at night which is one of the most difficult and dangerous times to drive. If an intoxicated driver injured you in an accident‚ you have a right to
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desired ligation product. We are trying to isolate the gene that encodes for bioluminescence. By varying the ratio‚ you have a higher chance of the target gene. b. Lane 1: HIND III standard Lane 2: L1/T0 Lane 3: L1/Tend Lane 4: L2/T0 Lane 5: L2/Tend Lane 6: L3/T0 Lane 7: L3/Tend Lane 8: L4/T0 Lane 9: L4/Tend Lane 10: L5/T0 Lane 11: L5/Tend c. The gel appeared to be in good condition with no breaks or tears. The gel did not seem to be punctured thus we were able to analyze the gel. Based on the
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Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
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of your SDS-PAGE analysis. 1 2 3 4 Figure 1. SDS-PAGE of purified protein X. Lane 1‚ Protein ladder (in Daltons). Lane 2‚ purified protein X (affinity chromatography). Lane 3‚ purified protein X (company manufactured). Lane 4‚ elution buffer. a. What is the benefit of a protein ladder/molecular weight marker in an SDS-PAGE gel? Describe what you can learn about the protein bands found in lanes 2‚ 3 and 4 based on the protein ladder bands. b. Positive and negative controls are absolutely
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because the author‚ Harlan Lane‚ was so specific and explained things through the thoughts she heard from people within the deaf community. In the beginning of the book‚ Lane discusses how the deaf culture views cochlear implants and the oral communication of deaf people. And though I was unsure why there was there was such a strong dislike of these things‚
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