STAINING FOR BACTERIAL ISOLATION Submitted by: Cherry Luz L. Rezaga Submitted to: Dr. Tomas J. Fernandez Jr. CRITERIA OF REPORTING 1. Thorough research (20%) 1.1. Number of literature cited more than five (5) 1.2. Completeness of the topic (5) 1.3. Principle discussed (5) 1.4. Literature should be cited in the text (5) 2. Confidence (20%) 2.1. Eye to eye contact (5) 2.2. No reading (5) 2.3. Proper handling of visual aids (5) 2.4. Magnified voice (5) 3. Appropriateness of visual
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THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during
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biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained it is called positive staining. It sticks with specimen and gives its color. Negative staining: If background
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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