Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
Premium Coomassie
What are the factors behind the rise of Mega Cities‚ and to what extent are these urban areas sustainable? Megacities‚ defined by population‚ have at least 10 million inhabitants. In recent years‚ megacities in the developing world have grown rapidly with little or no planning. As a result‚ they face cries of infrastructure‚ poverty and unemployment. The driving force for the rise of megacities is mass urbanisation between rural – urban areas‚ as well as rates of natural increase that are higher
Premium Population Metropolitan area City
laws of the land. When the founding fathers were drawing up the constitution‚ they had to put in mind for the future. They not only had to think about the future‚ but about individual of rights of the people. Limited government is just that putting a limit on how much power one should have without violating the liberty’s right. With limited government and people’s liberty in mind‚ the founding fathers had to think of a way to have the powers of government into a
Premium Political philosophy Law United States
Discussion Questions: 1. Should advertisers stay out of schools and respect them a “commercial free” zone? If not‚ what limits should they abide by in attempting to advertise to school-aged children and teenagers? “The methods used by advertisers seem limitless (Rae and Wong 338).” Commercial advertising—whether we agree to the extent that is goes or not—has become a major part of our culture. So we should not be surprised of how it is now breaching our schools and directly affecting the
Premium Advertising
Auschwitz was a concentration camp established in 1940 by the Nazis. The Nazis sent the Jews and other "undesirable" people here to be used as slaves or to be killed. It was located in Oswiecim‚ Poland. There was no privacy when the captives had to go to the washroom. Buckets and toilets were lined up in rows‚ and the toilets were often broken and flooded with urine and feces. Although the prisoners were exhausted due to hard work‚ sleeping was difficult because the bunks were made out of wood. The
Premium Nazi Germany The Holocaust Adolf Hitler
The Concentration of CO2 in the Atmosphere Introduction Carbon dioxide (CO2) is a colorless‚ odorless‚ non-flammable gas that can be produced by cellular respiration and burning of fossil fuels. Many types of industries such as breweries‚ mining ore and the manufacturing of carbonated drinks‚ drugs‚ disinfectants‚ pottery‚ and baking powder; utilize the CO2 gas (Appendix C). Recently Scientist proposed a theory in which CO2 concentration levels above 450ppm will lead to dramatic and potentially
Free Carbon dioxide
Most materials move by simple diffusion from high concentration to low concentration. Some substances may be actively transported‚ which requires energy. The diffusion of water across a semi-permeable membrane is called osmosis. Osmosis is the net movement of molecules through a semi-permeable [1] membrane from an area of low solute concentration to an area with a high solute concentration. In this investigation‚ we are testing how concentration effects the rate of osmosis Hypothesis: The more
Premium Concentration Osmosis
Introduction: This experiment was used to examine the hypothesis that: Osmosis is dependent on the concentrations of the substances involved. Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece‚ 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent‚ the other component‚ is any liquid in
Premium Osmosis Diffusion Concentration
PRINCIPLES OF MARKETING DMK0013 ASSIGNMENT 1 Topic: COMPANY CASE STUDY (AirAsia: The Sky’s the Limit) INSTRUCTION: 1. Answer the questions as per attached. 2. Mode: Individual 3. The format of the assignment shall be in accordance with the following description: Font : Arial Size : 11 Spacing : 1.5 Text : Justified 4. Assignment shall be at least 10 pages. 5. The cover page shall be in RED colour and the template is as per attached. 6. Ideas or texts
Premium Tony Fernandes Low-cost carrier Southwest Airlines
of bending. d) Calculate the maximum compressive stress in the beam‚ and show where it occurs along the length‚ and on the cross section. e) Calculate the maximum tensile stress in the beam‚ and show where it occurs along the length‚ and on the cross section. 2 kN/m 6 metres 3. The machine component shown below can be analysed as an overhung simply supported beam. It is made of cast iron‚ which is much stronger in compression than in tension. If the maximum stress must be limited to 30 MPa
Premium Force Compressive stress