Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe that both types of treated cells will express the beta-actin gene because it is a housekeeping gene. However‚ only HL-60 cells treated with PMA will express the MMP-9 gene by their RNA. The purpose of this lab is to view the effects of differentiation on protein synthesis
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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Name Class Date RNA and Protein Synthesis Information and Heredity Q: How does information flow from DNA to RNA to direct the synthesis of proteins? WHAT I KNOW 13.1 What is RNA? 13.2 How do cells make proteins? 13.3 What happens when a cell’s DNA changes? 13.4 How do cells regulate gene expression? WHAT I LEARNED SAMPLE ANSWER: RNA is a nucleic acid that carries coded genetic information. SAMPLE ANSWER: SAMPLE ANSWER: The bases in DNA—A‚ T‚ G‚ and C—form a four-letter “alphabet” that
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DNA is the key for our genetic apperance. DNA is a double stranded and is formed by blocks of nucleotides. Nucleotides each consist of three parts. A phosphate‚ a pentose sugar (deoxyribose) and a nitrogenous base. The nitrogenous bases carry the information for proteins which are needed in our body to function properly. Proteins are made according to the nitrogenous bases. When DNA replicates‚ it’s extremely important that it is copied exactly. If it’s not the protein which is produced according
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bacterial DNA replication Replication and transcription involves a parental DNA strand that is the foundation on which the products are built on. Replication and transcription both have initiation step which involve the breakage of the parental DNA strand. Replication and transcription both have specific proteins that keep the polymerase molecule attached to the parental DNA strand. There are elongation factors for transcription and sliding clamp for replication. Both processes use DNA topoisomerases
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lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included lysozyme‚ protamine sulfate‚ and bovine serum albumin‚ and the three non-protein samples contained either RNA‚ tyrosine‚ and glycylglycylglycine. Standard curves were created to exhibit the linear relationship between the concentration of solute (protein‚ non-protein)
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Background DNA is an abbreviation for deoxyribonucleic acid‚ but it is usually known by its initials alone. DNA is found in practically all living organisms‚ and it is now known to carry genetic information from one cell to the next‚ and from one generation to the next. The units of inheritance‚ called genes‚ are actually sections of the DNA molecule. Nuclei of the cells of higher organisms contain thread-like bodies called chromosomes‚ which consist of DNA‚ wrapped around proteins. So understanding how
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introns are removed‚ and if there are different amino acids produced during translation in the ribosome‚ then there will be unique proteins created‚ too. This allows there to be more types of proteins in an organism than types of genes in an organism’s genome. Repressor proteins can influence the regulation of protein synthesis by binding to operators (the segments of DNA that act as switches to either allow or prohibit RNA polymerase to create mRNA strands) in an operon. Since repressors block transcription
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use throughout the body. The liver is essential for monitoring and maintaining a relatively constant level of glucose in the bloodstream‚ hence the liver is involved in glycogen breakdown and synthesis because these pathways allow the liver to remove glucose from the bloodstream for glycogen synthesis when blood glucose is high and to release glucose into the bloodstream from glycogen breakdown when blood glucose levels are too low. Glycogen provides an additional source of glucose besides that
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