Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each
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Candy chromatography My experiment is called candy chromatography. This project is mainly about the colors that are mixed with other colors to make candy markers Koolaid and much more. I think that only the color of the candy is going to show up on the coffee paper. I think that threw the entire experiment the color in the candy is going to show up the entire time. I think this is going to happen because most of the colors are solid colors. The material and equipment used was a sample of candy
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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Purpose 1. To prepare a dry-packed sample of product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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