Learning Team Project Selection Ying Schwarte‚ Tonja Turner‚ Tina Dannenberg STR/ 581 Aug 19‚ 2013 Jacob Nichols Learning Team Project Selection Members in Learning Team D feel that reviewing one another’s paper and providing feedback to one another has been extremely helpful‚ and everyone has made great improvement in writing skills and critical thinking skills. It was difficult to select one or two projects‚ because each project has its own uniqueness. Nevertheless‚ Tina
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distillation and gas chromatography based on their difference in boiling points. The boiling point of hexane is 69 degrees Celsius and the boiling point of toluene is 110 degrees Celsius. Three fractions were collected for both simple and fractional distillation. The first fraction was hexane since it had the lower boiling point. The second fraction was a mixture of toluene and hexane. The third fraction was toluene since it had the high boiling point of 110 degrees Celsius. Gas chromatography was also preformed
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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Introduction In a chromatographic separation process‚ different kinds of functional groups and number of functional groups of molecules will determine the separation. Depending on what kind of solute‚ solvent‚ and adsorbent used‚ molecules will experience the interaction during dynamic equilibrium. On a TLC plate‚ capillary tube is used to transfer o- and p- hydroxyacetopheone‚ taking advantage of capillary force to make small spotting. A 30:70 ether acetate: petroleum ether is used as a solvent
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Encyclopædia Britannica Paper chromatography is used to separate "dissolved chemical substances". An example of dissolved chemical substances could be the pigments that make up a specific color‚ for example in a marker. When a chromatography paper experiment is done on a black marker‚ the experiment tends to separate all the different pigments that make up the color. In this experiment‚ the substance investigated was a black water soluble Giotto marker pen. Paper chromatography has two phases‚ the
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Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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Thin Layer Chromatography of Unknown Samples and Paprika By: Uyen Huynh Date: June 18‚ 2014 Lab Partners: Philip Murray Professor and section: M. Pandey‚ CHM2210L.601 Data: Analysis of Drug Store Items Knowns Rf Values Aspirin .91 cm Acetaminophen .85 cm Ascorbic Acid .86 cm Unknown .97 cm Identity of unknown mixture Acetaminophen Analysis of Paprika Pigments Spot Number Rf Color 1 1 Red-orange 2 1 Red-orange Calculations
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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Gas chromatography (GC) is a type of chromatography that uses a carrier gas as the mobile phase and a column as the stationary phase. The sample is injected into the instrument and is heated until the sample‚ which includes both analyte and solvent‚ boils. The analyte must have a relatively
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The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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