Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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Co(salen). The melting point of Co(salen) was138oC. Co(salen) was able to absorb 6.83x10-5mol of O2 Why? What chemistry is taking place? Introduction Heme proteins have been studied for years by scientists. Heme proteins contain a transition metal that can coordinate molecular oxygen. Examples of this can be found in myoglobin‚ hemoglobin proteins‚ copper containing hemocyanian and vanadium containing hemovanadin‚ these are all Essential for life. Myoglobin contains a porphyrin ring with an iron center
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membrane controls how and what substances can move in and out of the cell/organelle The structure of the membrane is often referred to as the “Fluid Mosaic Model”; this is because of the way it is structured It is composed of phospholipids‚ proteins‚ and carbohydrates‚ which are arranged in a fluid mosaic structure. The phospholipids are arranged in a “bilayer”. With their hydrophilic (water attracting) phosphate heads facing outwards and their hydrophobic (water fearing) tails facing in
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)? The percent acrylamide refers to the size of the pores as percent acrylamide increases the size of the pores decreases. 2. Describe the purpose of each loading buffer ingredient added to protein samples for SDS-PAGE analysis (hint- there are 4 ingredients). 3. You purified protein X via affinity chromatography (no diafiltration step performed) and ran an SDS-PAGE gel
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membrane (Zerboni et. al‚ 2014). It is predicted that viral envelope proteins interact with cell surface receptor such as manose-6-phosphate. After entry into the host cell the viron undergoes uncoating and protein 62 is produced. This protein is regarded as a transcription factor‚ therefore it must be produced and released before new protein synthesis can occur (Zerboni et. al‚ 2014). The virus codes for 3 immediate-early (IE) proteins that also play a role in regulating viral transcription by inhibiting
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Comparative Proteomics: Protein Profiler Lab by Jonathan Thulson Biology 113 October 6‚ 2013 Lab Partner: Vernon Morris INTRODUCTION Proteomics is the study of proteins. Their functions‚ interactions with other proteins‚ cellular locations and levels at which they are expressed. The purpose of this lab was to compare the proteins present in different species of fish to be able to determine which species of fish have the closest relation. This can be determined based on which
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membranes‚ which are lipid bilayers that contain proteins embedded in them. The regulation of passage through the membrane is due to selective membrane permeability - a characteristic of biological membranes which allows them to separate substances of distinct chemical nature. In other words‚ they can be permeable to certain substances but not to others. The movements of most solutes through the membrane are mediated by membrane transport proteins which are specialized to varying degrees in the transport
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functions. One of the main functions is to help in digestion. The largest solid organ in the body‚ situated in the upper part of the abdomen on the right side. The liver has a multitude of important and complex functions‚ including to manufacture proteins‚ including albumin (to help maintain the volume of blood) and blood clotting factors; to synthesize‚ store‚ and process fats‚ including fatty acids (used for energy) and cholesterol; to metabolize and store
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single-stranded‚ genome with negative-sense‚ unsegmented‚ enveloped RNA virus (Fig. A). The genetic data is packaged as a ribonucleoprotein complex in which RNA is tightly bound by the viral nucleoprotein. The RNA genome of the virus encodes five proteins. The five proteins are
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