CATALASE Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in reproductive reactions. Likewise‚ catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of molecules of hydrogen peroxide to water and oxygen each second. Catalase is a tetramer of four polypeptide chains‚ each over 500 amino acids long. It contains
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4.3 (b) To investigate the effect of pH on the rate of catalase activity Teacher Notes Apparatus required per class group of 24 students Chopping board 12 Knife 12 Weigh boats 12 100 ml Graduated cylinders 36 Boiling tube 36 Syringe 12 Electronic balance 3 Dropper 12 Timer 12 Labels 36 Thermometer 12 Water bath 3 Enzyme source eg. Radishes/celery Hydrogen peroxide Range of buffer solutions pH paper Washing up liquid Disposable gloves The apparatus
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reaction involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected. Controlling a number of factors which include human error‚ temperature change
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Rennet (pron.: /ˈrɛnɨt/) is a complex of enzymes produced in any mammalian stomach‚ and is often used in the production of cheese. Rennet contains many enzymes‚ including a proteolytic enzyme (protease) that coagulates the milk‚ causing it to separate into solids (curds) and liquid (whey). They are also very important in the stomach of young mammals as they digest their mothers ’ milk. The active enzyme in rennet is called chymosin or rennin (EC 3.4.23.4) but there are also other important enzymes
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metabolism. It is produced by photo-phosphorylation and cellular respiration and used by enzymes and structural proteins in many cellular processes‚ including active transport‚ respiration‚ and cell division. One molecule of ATP contains three phosphate groups‚ and it is produced by ATP synthase from inorganic phosphate and adenosine diphosphate (ADP). ATP is used is many organisms and also in different ways. Below are a few ways in which ATP is used. When muscles contract in any organism‚ it means muscle
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the
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Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity. This lab shows how the enzyme decomposes in hydrogen peroxide. Methods and Materials Refer to handout attached to the back of lab Observations Table 1: The mL of oxygen produced with increase of catalase 30secs 60secs 90secs 120secs 150secs 180secs Disks: 2 17ml 16ml 21ml 26ml 31ml 35ml Disks: 4 8ml 19ml 27ml 35ml 44ml 53ml
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Purpose Understanding how catalase activity is affected by pH is the purpose of this experiment. Introduction Enzymes play an important role in daily life because of the chemical reactions. Almost chemical reactions require the presence of enzymes to promote the metabolic process. They are known as the incredibly efficient and highly specific biological catalysts. Most enzymes are protein with the ability to enhance the rate of reaction between molecules. To catalyze a reaction‚ the enzymes
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the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports my hypothesis: At pH buffer 7 the enzyme (catalase) will work most efficiently
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Enzyme Catalase What Factors Affect Enzyme Activity Michelina Bartolotto Lab Biology 111B February 2‚ 2014 /media/common.studymode/studymode-upload/stm/files/e1b9a3d6adf94ca848b12159c31f11b0.docx INTRODUCTION Enzymes are proteins that function as biological catalysts (Perry‚ Morton 2007). They maintain the body’s stable internal balance‚ and without them life would
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