WATER/BUFFER LECTURES .....SOME NOTES YOU SHOULD HAVE TAKEN IN CLASS \wxNotesInClassBe. 1301124 8 and Things you should have done‚ or tried... = all problems! Gas pressure/concentration: 1 Atm = 760 mm Hg = 10 m H20 = 15 psi (psi is the industrial unit). O2 and man: upper limit/ lower limit Minimum: Without adaptation‚ man can not survive on top of Mt Everest = 8‚000M = 0.3Atm = height of jet liners= 0.06 Atm O2 Man starts being affected at 0.5 ATM air = 18‚000’ Maximum
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme.
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CHEM 113L EXP# 7: Buffer Preparation and pH Measurements Revised . AMB 7-2005 Introduction: Even in quite dilute aqueous solutions‚ acetic acid is very slightly ionized (it would approach 99% ionization only as the concentration approaches 0.0 M): HC2H3O2(aq) + H2O(l) Ka = 1.8 x 10-5. H3O+ + C2H3O2- In general‚ if the acid is not extremely weak‚ the pH of a solution of a weak acid is governed by the concentration of the acid and Ka. Under similar conditions‚ the pH of a solution of a weak base
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Abstract α-amylase was immobilized covalently on iron oxide magnetic nanoparticles. The synthesis of magnetic nanoparticles was done by the coprecipitation conventional method. The chemical composition and particle size of the synthesized particles was confirmed via X-ray diffraction. Tyrosine‚ Lucien and chitosan and glutaraldehyde were investigated to make a covalent binding between the iron oxide magnetic core and the immobilized enzyme. Immobilization using chitosan and glutaraldehyde show
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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BUFFERS By: Luis P. Bazan‚ RPh.‚ Ph.D. A buffer solution is a solution of: 1. A weak acid or a weak base and 2. The salt of the weak acid or weak base Both must be present! A buffer solution has the ability to resist changes in pH upon the addition of small amounts of either acid or base. Consider an equal molar mixture of CH3COOH and CH3COONa CH3COOH (aq) H+ (aq) + CH3COO- (aq) Adding more acid creates a shift left IF enough acetate ions are present 16.3 Which of the
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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Purpose: The Objective of this lab was to learn how to measure the pH (or acidity) of commonly known fluids‚ using the correct tools and procedures. To then use that data to document the changes noticed when mixing those same fluids and changing their respective pH levels. Materials: In order to conduct this experiment several pieces of equipment and other materials were needed. The first item was a graduated cylinder‚ which was used in order to measure out the precise amount of fluids needed
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Lab Report DIFFUSION AND THE EFFECT OF AMYLASE. Aim: We will interpret and explain the activity of an enzyme added to a solution. We will analyze reactions in different pH ranges. The starch solution will appear blue when mixed with iodine. But after mixing the enzyme amylase to the starch‚ the starches are broken down into simple sugars. Now‚ when the iodine is added‚ no color change exist. Instruments and materials used: 1.) Dialysis bags x2 2.)
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Honeydew’s report. Specifically‚ discuss what is missing and how the missing information should have been presented. First the PH measurements‚ Dr. honey dew does not explain how the PH device was calibrated or if the blood sample was added or mixed with anything prior to the measuring of the pH (like water‚ etc.). In regards to the absorption spectroscopy‚ the method by which the concentration was obtained is questionable. Was the concentration known before the experiment began‚ or was it found based
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