"Which ph buffer allowed the highest amylase activity" Essays and Research Papers

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    Preparation of buffer solutions 1. Activation buffer (Mixed Phosphate BufferpH 5.5) Solution 1: An accurately weighed quantity of 1.61 g of potassium dihydrogen phosphate was dissolved in sufficient deionized water to produce 100 mL of solution. Solution 2: An accurately weighed quantity of 35.81 g of disodium hydrogen phosphate was dissolved in sufficient volume of deionized water to produce 100 mL. Accurately measured volume of 96.4 mL of solution 1 was mixed with 3.6 mL of solution 2 to get

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    affects the rate in which Amylase breaks down starch into maltose. In this reaction starch is the substrate and maltose is the product. Amylase is an enzyme‚ Enzymes‚ also called catalysts‚ are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Equipment: Boiling tubes Timers/ stopwatch Starch Solution of Amylase – colourless

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    a catalyst for specific chemical reaction by converting a specific reactants into a specific product. The enzyme activity will also be affected by temperature and pH value. In this experiment‚ enzyme was placed in carbohydrate solution at different temperature to identify unknown carbohydrate solution. The enzyme that involved in the reaction is salivary amylase. The action of amylase on starch is with the mixture of IKI (iodine and potassium iodide test).Starch solution will have a blue-black colour

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    LAB PH

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    PH at the UN Alfredo Vidal Ceballos 7/10/2014 Group Support Part 1: Solution Group Name and M. cons. Chem formula Type Experimental pH 1 Deidre Acetic Acid 0.1M HC2H3O2 Weak Acid 3.03 3.03 2 Acetic Acid 1M HC2H3O2 Weak Acid 2.59 2.54 3 Hydrochloric Acid 0.5M HClStrong Base 0.85 0.90 4 Jonah Nitric Acid 0.1M HNO3 Strong Acid 2.14 2.14 5 Sodium Acetate 1M NaC2H3O2 Weak Base 9.42 9.42 6 Potassium Dihydrogen Phosphate 0.1M KH2PO4 Weak Acid 5.47 5.47 7 Andy Potassium Hydrogen Phosphate 0

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    pH and its meaning

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    pH pH means Power of Hydrogen or Potential of Hydrogen. pH is a scale of acidity from 0 to 14. It tells how acidic or alkaline a substance is. More acidic solutions have lower pH. More alkaline solutions have higher pH. Substances which are not acidic or alkaline i.e neutral usually have a pH of around 7. Acids have a pH less than 7. Alkalis have a pH greater than 7. There are a lot of ways for finding the pH of something. One way is to use litmus paper. The pH paper is able to tell how strong

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    Ph Lab

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    of solutions and test their pH. Due to materials available pH strips were the only testing tools available. A table is setup to compare the strip color to its pH level. Four known solutions including bleach‚ lemon juice‚ soda‚ and baking soda were used. There was also an unknown solution in which the pH level was used to determine which solution it was. The pH scale is 1-14‚ a pH of 7 is neutral‚ less than 7 is acidic‚ and greater than 7 is acidic. Materials: * pH strips * 20 test tubes

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    BUFFER STOCK SCHEMES

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    BUFFER STOCK SCHEMES The prices of agricultural products such as wheat‚ cotton‚ cocoa‚ tea and coffee tend to fluctuate more than prices of manufactured products and services. This is largely due to the volatility in the market supply of agricultural products coupled with the fact that demand and supply are price inelastic. Products with unstable conditions of supply and demand will experience price fluctuations. Agricultural (farm) prices tend to be volatile because: Supply changes because

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    Soil pH Measurement

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    Experiment 3: Soil pH measurement Introduction Aims The aims of the experiment were to determine the pH of a variety of soils which included sedentary‚ 3:2‚ sand‚ organic matter with the means of a pH meter at various soil water ratios and with or without calcium chloride. Method and Materials Samples of sedentary soil‚ 3:2 soil‚ sand‚ organic matter and compost‚ 100ml vials(x12)‚ bottle of distilled water‚ analytical balance‚ pH meter‚ 2 buffer solution of known pH‚ 0.25M calcium chloride

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    Bases‚ Buffers and Respiration Darlene E. McDonald WCCC Fall 2012 Ap Lab 071-07 Dr. Roxanne Levandosky August 31‚ 2012 I. INTRODUCTION: A.PURPOSE: a. To define and understand the key terms. ~acid ~buffer system ~base ~acidosis ~pH ~alkalosis ~pH scale ~hypoventilation ~pH indicators ~hyperventilation b. To determine the acidic or base nature and actual pH of various substances. c. To discuss the formation of the carbonic acid/bicarbonate ion buffer system

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    Carbohydrates in relation to Ph. levels 2012 HAUBER-SIX‚ Janina Queensland Government 1/1/2012 Digestive System Enzymes Digestion of Carbohydrates in relation to Ph. levels 2012 HAUBER-SIX‚ Janina Queensland Government 1/1/2012 The follow experiment was prepared‚ conducted and analysed in order to test at which pH level starch will be digested at fastest. The digestive system is a complex network of organs as well as other structures which all work together to supply

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