DNA to pGEM genomic DNA using a phenol chloroform purification method. 2. Vibrio fischeri DNA and pGEM DNA was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared for us so that transformation of the recombinant plasmids and the competent cells through heat shock transformation. 5. The transformation was then plated on LB/amp/X-gal plates. 6. The plates were screened for our
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Bharti Airtel Group 2 • • • • • • • • • • • Ashish Dubey Dibyashree Mohanty Akshay Shetty Preeti Basu Anshul Kumar Ashish Kumar Mudit Arora Nikhil Kumar Rahul Sangtani Tribhuvan Sharma Puja Katare Vision‚ Mission and Values.. VISION MISSION VALUES By 2015 Airtel will be the most loved brand‚ enriching the lives of millions “ We at Airtel always think in fresh and innovative ways about the needs of our customers and how we want them to feel. We deliver what we promise and go out of our way
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Chapter 10 Section 404 Audits of Internal Control and Control Risk Review Questions 10-1 Management typically has three broad objectives in designing an effective internal control system. 1. Reliability of Financial Reporting Management is responsible for preparing financial statements for investors‚ creditors‚ and other users. Management has both a legal and professional responsibility to be sure that the information is fairly presented in accordance with reporting requirements
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The organizations mission is “To bring inspiration and innovation to every athlete in the world”. According to the founder Bill Bowerman “If your have a body‚ you are an athlete.” Nike is a global marketer of athletic footwear apparel and equipment. The organization operates in over 160 countries while also having whole owned subsidiaries which include Cole Haan‚ Converse Inc.‚ Hurley International‚ and Umbro. Nike focuses most of their marketing resources to find out consumers’ behavior when it comes
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Order Under provisions of Section 37 of the MHA‚ a person convicted of an imprisonable offence could be eligible for a hospital order. This order would give the authority for him to be admitted to a specified hospital‚ where he would be detained under the conditions as set by the Act‚ for treatment. Under the 1983 MHA the Courts were bound by the four categories as described by the Act‚ and they required to be satisfied‚ based on expert evidence‚ that the individual was suffering from one of the
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bussinesses and educational institutions to new heights. Before the advent of computers and relevant technology‚ business and information management were totally done using manual resources. As a result‚ the time taken to complete a task was more‚ quality of work was not up to the mark‚ and the procedures also tended to be more complicated (Rampur‚2010). However‚ as computers started to be used in business establishments‚ the processing of work got more stabilized. These technologies are used in almost
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Fides Bucu ENG 102 Essay Assignment # 2 Summary and Response Essay to Chapter 2 Switzerland Weiner reaches and visits different places in Switzerland via train which he falls in love with and thinks that it is punctual and efficient. He talks to various Swiss to find out why they are happy. However‚ they are surprised to hear why he thinks they are happy. They state that Swiss do no want to “put themselves in the spotlight”therefore not provoking envy among others. They do not talk about money
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According to Fremgen (2012)‚ every state has a statue or regulation that necessitates that all health care professionals and physicians report all cases of acquired immunodeficiency syndrome (AIDS) to the department of health local or state level. This would allow Dr. K to meet his obligation in reporting the communicable disease. In some states it is also legal to inform a spouse‚ needle sharing partner‚ or other party that may be at risk of contracting the disease (Fremgen‚ 2012). In this case
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E1: Describe three different types of settings which provide care and education for children in your area. Bracebridge Infant and Nursery School: This is a statutory school set up and funded by the government. The governors within the school have a key role in managing the finances‚ recruitment and ensuring Bracebridge Infant and Nursery School provide a high level of education for all it ’s pupils. The school encourages academic‚ social and emotional development. Our Lady of Lincoln Catholic
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IAS 2 Inventories This version includes amendments resulting from new and amended IFRSs issued up to 31 March 2004. The section “Changes in this Edition” at the front of this volume provides the application dates of these new and amended IFRSs and also identifies those current IFRSs that are not included in this volume. International Accounting Standard 2 Inventories (IAS 2) is set out in paragraphs 1-42 and the Appendix. All the paragraphs have equal authority but retain the IASC format of the
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