1. Sequence A TCT TCC CTC CTA AAC GTT CAA CCG GTT CTT AAT CCG CCG CCA GGG CCC CGC CCC TCA GAA GTT GGT Sequence B TCA GAC GTT TTT GCC CCG TAA CAA CTT GTT ACA ACA TGG TCA TAA ACG TCA GAG ATG GTC AAT CTC TTA ATG ACT Sequence C TAC AAA CAT GTA AAC ACA CCC TCA GTG GAC CAA CTC CGC AAC ATA AAC CAA ACA CCG CTC GCG CCG AAA AAG ATA TGG 3. 4. Sequence A - Middle Sequence AGA AGG GAG GAU UUG CAA GUU GGC CAA GAA UUA GGC GGC GGU CCC GGG GCG GGG AGU CUU CAA CCA Sequence B - End Sequence
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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Friends can Lift You Up or Bring You Down “Hey whatz up bro? How’s life? Today there’s a party at my house” These are some of the common conversations in our daily life. Friends here‚ friends there‚ friends everywhere. Life without friends is like garden without flowers. Friends are one of the most valuable possessions in life. Friends are those people who will always be with you in the ups and downs of your life. Friends help us in many ways. They always comfort us when a problem arises‚ which
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Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively
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Capital Punishment: An Eye for an Eye For many years there has been a constant debate between supporters and non-supporters of the death penalty. Many people argue that the death penalty is as much a murder as the crime committed by the offender. Others may say that it provides closure and justice. I am a strong supporter of the death penalty. There are many others like myself. The world is full of the most dangerous and ruthless criminals that should be put to death. There are many pros that the
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History 1301 Why you should not smoke Have you ever been walking into a store and a pungent smell slaps you across the face? You turn around only to see that someone is smoking a cigarette. Have you ever wondered the affects that smoking would cause? How would smoking affect a person’s appearance and health? Have you ever wondered just how many people smoke worldwide? According to the CDC (Center for Disease Control and Prevention)‚ about 43.8 million US adults (21.6 percent of men and 16.5 percent
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electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel matrix. In visualization‚ the gel is dyed so the results become present. This is used in modern crime labs‚ figuring out DNA‚ which plays a key role in many criminal trials. The researcher completed this experiment to figure out who committed the murder using gel electrophoresis. The researcher followed five steps in this experiment; First was placing the gel in the electrophoresis
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acid‚ or DNA as it is most commonly known‚ is a strand of molecules found within the cell nucleus of all living things. It is called a “genetic fingerprint” because each is dissimilar to the other and each person‚ apart from identical twins‚ has different DNA patterns. DNA testing has overthrown the way law enforcement agencies collect evidence in numerous criminal cases‚ especially rape and murder and consequently had a large impact on countless past cases. The prospect of a national DNA database
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Strayer University CRJ 320 Criminal Investigation Professor: Elaine Wilson The importance and legal uses of DNA evidence in Criminal Investigations Tasha L. Thomas 4/21/2013 Once a crime is committed law enforcement officers are dispatched to the scene and a criminal investigation begins immediately. The first responder to the scene begins to taper off the area and assures that nothing is touched or risk of contamination of evidence occurs‚ while waiting on the
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When DNA profiling was first used in criminal cases‚ it was often difficult for prosecutors and defense attorneys‚ as well as the experts they hired to testify‚ to explain the significance of their DNA match to the jury. Fingerprints are still considered by most people to be an ironclad way to identify someone‚ but an expert testifying about fingerprints discusses them in terms of "points of similarity." DNA matches are discussed in terms of statistical probability using what is currently known about
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