Determining the Concentration of an Unknown Protein Solution with the use of the Bradford Method Paul Alcala‚ Andrea Basa‚ Melissa Caagbay‚ Frances Cayomba Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state
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Title: Bradford Assay: Creating a Standard Curve and Determining Unknown Protein Concentrations Introduction: In biochemistry the ability to determine the quantity of a certain substance in a solution is extremely important. Often protein concentration within a biological organism is essential in determining the function or condition of that organism. If the protein concentration in an environment is increased or decreased it can have detrimental side effects. If there is a decrease in hemoglobin
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William Bradford and John Smith‚ two different guys but at the same time accomplished many things throughout their lives. They were similar in a couple ways but there were also things that distinguish them. Both‚ John Smith’s persuasive pamphlet and William Bradford’s Of Plymouth Plantation present a different picture of the same pre-colonial land of New England. In addition‚ they established colonies and through their writings‚ they hoped to attract more settlers with similar cases. Some things
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On November 11‚ 1620 the pilgrims‚ aboard the Mayflower ship‚ dropped anchor at Cape Cod. They wanted to explore the new land with a shallop they brought with them from England‚ but it suffered damages during the voyage and needed mending. In this situation‚ while the shallop was being repaired‚ 16 well armed men‚ leaded by Captain Standish‚ were allowed to seek what seemed to be a nearby river. This was unknown land‚ so they took every precaution available to them. On the 15th of November they
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Writers are characterized by three factors. These factors are style‚ tone‚ and purpose. William Byrd and William Bradford were two colonial writers however they took completely opposite approaches toward writing. During these times‚ journals‚ diaries‚ and sermons made up the literature. Byrd and Bradford were no exceptions with their works of A History of the Dividing Line and Of Plymouth Plantation respectively. Whether it was the difference in writing styles‚ the different purposes for writing
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Bradford Protein Assay Practical Report 1. Present your data (including raw data and calculated concentrations) for the protein standards in the form of a clear table. Give one example of how you calculated protein concentration. Do not forget a descriptive title and units (4marks) Title either too long or not descriptive or absent Your results are in duplicate & shouldn’t be referred to as ‘set1 & set2’ or ‘original’ and ‘duplicate’ Many of you think units of absorbance are nm but A has arbitrary
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versus the Puritan view William Bradford was the leader of one of the earliest colonial settlements in the United States‚ of which Thomas Morton was a settler. Bradford documented his exploits in “Of Plymouth Plantation” to gain support from his home country and fellow colonists. Bradford’s work‚ however‚ differs from Morton’s “New English Canaan” which also describes the events which took place in the colonies and his views of the Cavaliers‚ the Indians‚ and the Pilgrims. Bradford applies his
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Sam Bradford Samuel Jacob Bradford was born November 8th of 1978 to his loving parents‚ Martha and Kent Bradford. Sam is the only child‚ which allowed his parents to devote all their time to him and support what it is he wanted to do. Whether it was playing sports or the cello‚ his parents did what they could to develop it. They encouraged him in everything. "As long as it was a good‚ wholesome thing‚” Kent said. "If he wanted to do it‚ do it.” Even though Sam was central to their lives‚ he wasn’t
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Puritans started to arrive in the New England area during the 1600’s. They specifically settles in the areas around Boston. Unlike other people coming to settle in New England‚ the Puritans came to create a more pure and Christian based society; they did not come for economic purposes. The Puritans wanted a theocratic society‚ or a government run by religious beliefs. By creating their Christian based society in this way‚ their ideas and values of the political‚ economic‚ and social developments
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The Puritans are a well known religious group. They were known for reasons that made them separate from the Catholic Church. They are also extremely well known for their strong work ethic. This ethic was the foundation of America and is now lost upon the Americans who depend upon their country to give them the material possessions they want but do not want to do any work for those things. A large number of people who came over to America from England in the 1600’s were Puritan. They moved to American
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