Based on the data from the riboflavin spectrum scan‚ the maximum absorbance wavelength for this compound is 446 nm. This was the point between 390 nm and 500 nm at which the absorbance value (0.72) was the highest. A blank tube that has the components of the solution being examined except for the compound of interest is then used in combination to provide an even more accurate reading. Then‚ by using Beer’s Law‚ the molar extinction coefficient for riboflavin was able to be calculated: 14‚400 L/(moles*cm)
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The characteristic properties of slime can be explained by polymerisation reactions. Polymers can be explained most simply by the polymerisation of the molecule ethylene into polyethylene (see figure 1 below). Figure 1. Polymerisation of polyethylene from ethylene. In this example‚ the double bond between the carbon atoms is broken‚ allowing each carbon atom to form one more bond between another molecule. The double bond between the carbon atoms is weaker because the type of orbital is ‚ compared
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The first step was to prepare 400 mL of an 0.1 M solution of NaOH. This was done by diluting from the 6 M solution that was provided. Next‚ 0.715g of KHP was weighed using the glazed paper and the triple beam balance. The KHP was then transferred to the 250 mL Erlenmeyer flask. Using a graduated cylinder‚ 50 mL of deionized water was measured and added to the flask. The KHP was dissolved in the water‚ and few drops of phenolphthalein were added. Moreover‚ the burette was rinsed with deionized water
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Specimen Preparation: Samples and all test reagent and kit were brought into room temperature. In 2ml eppendrof tube approximately 220mg stool sample were taken. Washing buffer was prepared by adding distilled water . Procedure for Purification of DNA from Stool sample: i. 220mg stool sample were collected in a 2ml tubes and placed it on ice. ii. Added 2ml Buffer ASL to each stool tube. Used pipet to wash the stool sample from the spoon while transferring the buffer. Vortexed continuously for 1minute
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Throughout the data and the observations got from this experiment‚ there are some reasons which may affected the mass of the copper was recovered and also the percentage of yield. According to the law of conservation of matter‚ the mass of the copper will not change even though there are many chemical reactions happened which also mean that the mass of copper contained in solutions or precipitates remain the same as the copper in the beginning. And it is necessary to synthesize the various copper
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This experiment consisted of two parts. First‚ the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next‚ the purple Gatorade extraction procedure was carried out‚ beginning with pipetting 1 mL of 70% isopropanol
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The purpose of the first week experiment was to determine the effect of Nitrophenyl phosphate concentration‚ the substrate‚ on the enzyme‚ acid phosphatase‚ reaction. In an enzyme reaction‚ there is substrate that binds to the active site of the enzyme and product is the end result. The enzyme is the catalyst for the reaction which means that it speeds up the process. Without enzymes‚ the majority of reactions would not occur because the activation energy level would be unattainable. With this in
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The lipase gene sequence from Alcaligenes sp. JG3 was translated into amino acid sequence using two methods‚ manual translation and ExPASy online software. By manual method‚ amino acid sequence was obtained via codon translation one by one as shown in Figure IV.7. The sequence reference used is the lipase from A. faecalis MOR02 due to having high similarity with the lipase sequence from strain JG3 during CLUSTAL alignment and the alignment of the deduced amino acid with the referred sequence was
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In this section we will analyze which steps were the most effective ones in recovering LDH (percent yield) and in purifying LDH (fold purification). As we can see looking at the Total Protein column on Table 3‚ the most effective step with regard to the percent of remaining protein removed was affinity chromatography because it was able to remove 98.6% of the remaining proteins. In comparison to 81.93% removed during the 65% ammonium sulfate precipitation and 81.3% during the size exclusion
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1. What is the more formal chemical name for aspirin? Acetylsalicylic acid 2. Using your data from Experiment 2‚ how can you be sure you synthesized aspirin? By using the colors as a guide‚ we can compare the Acetylsalicylic acid + 8 mL FeCl3 to the Aspirin Crystals from Experiment 1 + 8 mL FeCl3 in order to see if it synthesized. 3. Would you say that your synthesized aspirin is relatively pure? How can you tell? One way that the purity of aspirin can be judged is by it’s color as pure
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