In the experiment‚ 0.3 g of mixture was dissolved in 3 mL of t-butyl methyl ether in a centrifuge tube. Then‚ 2 mL of 0.5 M NaHCO3 was added‚ shaken for one minute‚ and allowed to sit for two minutes so the layers could separate. The lower water layer was removed with a Pasteur pipette and placed in a test tube labeled “Acid.” Next‚ 0.5 mL of distilled water was added to wash the ether layer in the centrifuge tube and then discarded. 2 mL of 0.5 M NaOH was added to the remaining layer‚ shaken for
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Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
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Associate Level Material Appendix D Disaster Recovery Plan Student Name: Christopher McCleary University of Phoenix IT/244 Intro to IT Security Instructor’s Name: Mr. Romel Rausa Llarena Date: 4/15/12 Disaster Recovery Plan Due in Week Three: For your selected scenario‚ describe the key elements of the Disaster Recovery Plan to be used in case of a disaster and the plan for testing the DRP. 1 Risk Assessment 1 Critical business processes
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Cheyenne Pruett Ms. Mitchell College Composition October 8‚ 2013 Ophelia? Thomas G. Plummer‚ in his essay “Diagnosing and Treating the Ophelia Syndrome” argues that the system we use for teaching today takes away students ability to think for themselves. Plummer proves his argument with loads of examples of students conforming to others to take the easy way out. In Plummers essay he points out ways to treat this syndrome and stop all ignorant people from ruining the minds of other
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Music History Review: Romantic The Norwegian composer Edvard Grieg penned in a letter that he wrote to his friend Gottfred Mattison-Hansen on July 30‚ 1867‚ his second violin sonata “in the euphoria of my honeymoon”.1 This sonata in G major was a labour of affection that the composer wrote in honour of the love of his life Nina Hagerup‚ who was his cousin‚ whom he married despite familial disapproval on both sides. The evident happiness is reflected in the demeanour and beauty of the work
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A G Barr plc — a case study Background information A G Barr plc manufactures‚ distributes and markets drinks‚ primarily carbonated soft drinks. Based in Glasgow‚ it has been manufacturing soft drinks in Scotland since 1875. Its most famous product‚ Irn-Bru‚ was first produced in 1901. Barr’s became a public limited company in 1965. The business has always been associated with the Barr family and members of it own the majority of shares in the company. However‚ Robin Barr‚ the
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Welcome to WritePoint‚ the automated review system that recognizes errors most commonly made by university students in academic essays. The system embeds comments into your paper and suggests possible changes in grammar and style. Please evaluate each comment carefully to ensure that the suggested change is appropriate for your paper‚ but remember that your instructor’s preferences for style and format prevail. You will also need to review your own citations and references since WritePoint capability
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Scenario: John is an 18-year-old freshman at New York University. John was the starting quarterback at his high school in a small town in Indiana. He enjoys playing sports and hanging out with friends. John has always been popular and done well in school and sports. Once he is comfortable in his surroundings he can be confident in what he is doing. Donnie is an 18-year-old freshman from Chicago attending Iowa State. Donnie spends most of his time playing his guitar and writing music. In high
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Inventory Optimization at Procter & Gamble: Achieving Real Benefits through User Adoption of Inventory Tools Procter & gamble (P&G) is a leading global consumer products company founded in 1837 with $76.7 billion in sales in 180 countries. P&G is famous for their brand names like Tide‚ Gillette‚ Pantene and many others. It competes in 26 distinct product-category markets such as hair care‚ cosmetics‚ paper towels‚ skin care‚ oral care‚ blades and razors‚ diapers and fabric care. Procter &
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season Strategy for 2 in India Brand Strategist The Dream Team Season 2 Case Study for Strategy Development S T R AT E G Y F O R T I D E I N I N D I A Case Background The Mint‚ 3rd December 2012: In a recent global investor meeting‚ Procter & Gamble affirmed that a strong momentum in developing markets continues to be a key part of its strategy. It said it will invest resources on the biggest opportunities‚ and aim for profitable expansion by focusing on growing markets‚ increasing market
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