magnifier we can see single bacteria dark cell. B: On each examples bacterial morphologies are visible and easy identified. In the wet mount example with yeast‚ cocci is noted‚ while is hard to determine in cheek smears. Check smear and yeast smear contain cocci with direct staining. At direct staining plaque smear contain cocci and bacillus. Yeast with indirect staining contains cocci as well. On same picture bacteria are singled while on some pictures bacteria are in group and uneven clusters. Different
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allowing the bacterium to become directly stained. In direct staining‚ the organisms must be fixed by a process such as heat. Fixing the slide prevents the organism form washing off the slide before visualization. This is accomplished by passing a smear of the bacteria through flame. The heat sets the proteins of the organism thus causing the bacteria to attach to the slide. The organism can become damaged from the setting process and the use of heat prior to staining. In indirect staining‚ the negatively
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stained and unstained organisms. A wet mount is a preparation process where a live specimen in culture fluid is placed on a slide and the organism is free to move about. In the wet mount slides provided via LabPaq software with cheek‚ dental plaque‚ and yeast specimens were observed. The wet mount preparations were difficult to observe because of poor contrast‚ however‚ a common occurrence in the specimens were cells large in size and translucent in color. The slides provided with direct staining
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at chains of cocci or bracillus. Part 5: Indirect Staining: Examine the stained specimens and record your results. These were much easier to see‚ especially the plaque stain. I saw distinct chains of varying lengths made up of both cocci and bracillus cells. The messy matrix I noted earlier was no longer an issue. The cheek and yeast smear were also much cleaner. I saw the same shapes as before‚ just with sharper outlines. Questions: A. What are the advantages of using bleach as a disinfectant
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cell. Slide Three: This slide was a mixture of different shapes. Nothing was recognizable. Part 5: Indirect Staining: Chains of both cocci and bracillus cells were both visible and identifiable. The chains varied in length. The cheek and yeast smear was clearer. The same shapes were seen as before just with sharper outlines. The cells were much easier to see with more detail. Questions: A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages
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Procedure – Instructions followed as per procedure‚ specimens viewed through v-scope‚ using 10x‚ 20x‚ and 40x stained wet-mount. Observations compared amongst one another from cd-rom. Spirillum bacteria Bacillus Cheek Swab Yeast Analysis – Using the virtual microscope‚ the images seemed to be more clear and concise. Using the light microscope again for another experiment has increased my confidence in its utilization. Although the procedure was different‚ the experiment
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Labset One Worksheet 1. Label and describe the function of each of the following parts of the microscope (7) A. The ocular lens/eyepiece transmits and magnifies the image from the objective lens to the eye. B. The body tube transmits the image from the objective lens to the ocular lens. C. The nosepiece is a rotating mount holding many objective lenses. D. The objective lens is the primary lens that gathers as much light as possible from the specimen and brings it to a bright focus
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bacillus‚ diplobacillus‚ streptobacillus. G. Were you able to identify specific bacterial morphologies on either yogurt slide? If so‚ which types? I believe I could see cocci‚ and diplobacillus. H. Describe the cells you were able to see in the blood smear. Red blood
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MBK – Lab Report Name: ____________________ Section: ___________________ Bacterial Morphology Pg 61 Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining:
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Bacterial Morphology Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained
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