of the slide with a 45 degree angle. Remove some of the excess stain that’s on the slide using a tissue paper. To finish‚ view your slide on the COMPLEX STAINING microscope. Clean and dry microscope slides thoroughly. Flame the surface in which the smear is to be spread. COMPLEX STAINING 3. 4. 5. 6. 7. 8. Flame the inoculating loop. Transfer a loop full of tap water to the flamed slide surface. Reflame the loop making sure the entire length of the wire that will enter the tube has been heated to
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Methods: Simple Stain For this experiment‚ I used E. coli to make a heat fix smear. I placed a drop of water in middle of the slide‚ then put in small amount of E. coli and mixed well. The slide was allowed to air dry. When it was completely dry‚ I passed the slide over the flame for 3 times to fix the cells to the slide. For the staining part‚ I put a drop of methylene blue onto the smear for 30 second‚ rinsed with water‚ blotted dry and observed the slide using oil immersion.
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1320 HBL for CP2078 (Sample Preparation for Microscopy) Name of students Admission No Class Total Marks / 45 Assignment 1 1. Explain the principle of positive staining. (3 marks) Positive staining is done by staining the cell walls of Gram-positive bacteria with crystal violet. The cell wall of Gram-positive bacteria is made up of a thick layer of peptidoglycan which undergoes dehydration during decolourisation causing the pores to shrink
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Sc KEY STAGE 3 TIERS Science tests Mark scheme for Papers 1 and 2 2009 3–7 National curriculum assessments 2009 KS3 Science Mark Scheme Tiers 3–6 and 5–7 Introduction ________________________________________________________________________________________________ Introduction This booklet includes the mark scheme for paper 1 and paper 2 in both tiers. The structure of the mark scheme for tiers 3–6 and 5–7 The mark scheme for each question shows: I the teaching
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respectively. Only 19.2% had undergone a clinical braest examination within one year and 3.6% had ever undergone a mamography. Only 76.3% knew that a Pap smear detects precancerous stage of cervical cancer. Among 169 married workers‚ 73.4% had never had a Pap smear and only 17.2% had got it done within the preceding 5 years. Among the reasons for not doing a pap smear within 5 years‚ 47.0% belived it as not nescessary‚ 17.3% due to fear/dislike‚ 23.2% as not having symptoms‚ 3% had not known about it and 3%
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Refer to SLUPPMD unity for sputum AFB smear ↓ PTB Yes No ↓ ↓ Treat Refer to TBDC ↓ Send back to referring physician - Importance of taking the drug - Role of treatment
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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Methods Smear Preparation Obtained a clean dry glass slide‚ Staphylococcus aureus and Pseudomonas cultures. Added drop of water onto the center of slide. 3. Flame-sterilized inoculating loop‚ top of culture tube‚ obtained a very small sample of bacteria and smeared onto the water drop on the slide slide Repeated smear with second bacteria culture tube. Smear was allowed to air dry and was subsequently heat-fixed onto the slide. Gram Stain The S. aureus and P. fluorensens smear was covered
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from the slant S. aureus into the drop of water. Mix both cultures on the slide with an inoculating loop to make a mixed smear. Air dry and heat fix this smear. Cut a sheet of bibulous paper to fit the dimension of the slide and place this paper on top of smear. When water is boiling‚ place the paper-covered slide on top of the beaker. Add carbolfuchin to the paper covering the smear. Steam the preparation for 10-12 minutes. Do not let the paper dry out. Add more carbolfuchin to keep the paper wet while
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Ms. Jenette Malaban Activity 6: Fecal Analysis for the Detection of Parasites I. Objectives: 1. T o perform the basic techniques in fecal and blood analyses. 2. To identify parasitic forms that are recognizable in the fecal samples and blood smears by microscopy. 3. To determine the advantages and limitations in each procedure. II. Methodology Before the activity of checking or detecting the presence of parasites to the stools‚ it was started with getting a stool samples from the grade
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