Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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The experimental membrane we used in this lab acted as a barrier in which only a small number of molecules can pass through. In this experiment we wanted to show the process of osmosis‚ in which water passes through the aforementioned barrier. Osmosis is critical to life because the process regulates
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Name Lab‚ Week #1 BALANCE LAB Introduction Several instruments are used for determining the mass of a chemical; these instruments are called “balances.” However‚ different balances have different measurements‚ meaning that some balances are more accurate than others. Moreover‚ there are several techniques that are used or practiced in order to obtain an accurate measurement. The two techniques are direct weighing and weighing by difference. Direct weighing simply means to read the mass
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Lab 9 Working with Printers Exercise 9.1 Installing a Printer Overview Contoso‚ Ltd. has just taken delivery of several new printers that the IT director purchased through an auction. He has assigned you the task of installing the printers and making them available to the users of the company network. For the first printer‚ you intend to connect the unit directly to an LPT port in the Windows 7 computer that will function as the print server. In Exercise 9.1‚ you install the driver for the printer
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Adwoa Fosuah Bio Psych Lab Report 4 Cold Pressor Task Aim The cold pressor test is done by having the subject submerge their hands in a bucket of cold water to determine cardiovascular changes such as blood pressure and heart rate. For this experiment we wanted to know how long participant can keep their feet in the bucket of ice water before they experience any pain while looking at neutral and positive pictures and also rate the intensity of their pain. For this reason we hypothesized that
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of Oxalate Ion in Ferric Oxalate Trihydrate using Titrimetry Abstract: In this two-part lab‚ we will learn about coordination compounds and their uses with stoiciometry. We will also find out about how theoretical yield is calculated from a reaction we will create. We will also synthesize Potassium Ferric Oxalate Trihydrate (K_3 [〖Fe(C_2 O_4)〗_3]•3H_2 O) using a two step reaction. In the second part of this lab we will calculate how much Oxalate Ion is present in the K_3 [〖Fe(C_2 O_4)〗_3]•3H_2 O using
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Date of Experiment: September 10th‚ 2013 Organic Chemistry II – CHLB330 Name: Symone E. MoxeyLab Partner: Lynden Cooper Synthesis of Aspirin (Acetylsalicylic Acid) Abstract:- Aspirin (acetylsalicylic acid) is produced experimentally in the lab. The resulting percentage yield is 65.5%. The purity of the obtained product is tested using the melting point and Ferric Chloride Test (FCT). The aspirin was massed‚ and the melting point was determined. Based on the data collected‚ there was a total
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Lab Report Problem: What method would consistently test the foam and ensure it has the correct density? Background: There are words that need to be understood before executing the experiment. The first thing you need to know is density. Density is a measure of mass per unit of volume. The reason for testing density of doors because doors must contain a density of 14.34lbs per ft3 of foam‚ or it will collapse if the density is too high. If the density is too low‚ it won’t allow for normal expansion
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Flame Test Laboratory Report Introduction The purpose of this lab is to observe and identify metallic ions‚ using flame tests. The traditional flame test originated when Robert Bunsen invented the Bunsen burner to test two new alkali metals he and his partner had discovered. “A flame test is an analytic procedure used in chemistry to detect the presence of certain elements‚ primarily metal ions‚ based on each element’s characteristic emission spectrum” (Flame Test 1). Using this test‚ an element
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Lab 17 Amino Acids and Proteins Lab date 10/22/2013 12-1350 I Purpose The purpose of this experiment was to separate mixtures of II Method For a complete list of experimental procedures see prelab outline attachment #1. “For a complete list of experimental procedure see Seager‚ Spencer L. and Slabaugh‚ Michael R. Safety-Scale Laboratory Experiments for Chemistry for Today General‚ Organic and Biochemistry; Thomson Brooks/Cole‚ Belmont‚ CA‚ USA‚ 2008; pp.221-225”. III Data Part A Mass
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