1.1.5 Elisa Testing
1.,1.5
Activity 1.1.5: ELISA Name _______________________Per_____ |
Summarize the steps you took to conduct the ELISA test.
Conclusion 1. Explain why antibodies allow scientists to target and identify specific disease agents. * * * * * * 2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? * * * * * 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? * * * * 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. * * * * * * 5. Why do you think college students living in dorms are often populations who see meningitis outbreaks? * * * * * 6. How did ELISA data allow you to track the path of infection at the college? * * * * * * 7. Discuss the limitations of using antigen concentration to deduce the path of infection. Be sure to refer to the workings of the human immune system. * * * * * * 8. The ELISA test can also be used to detect antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment, outline a procedure for testing for antibodies in the blood. * * * * * * 9. Explain why in sudden outbreaks, it may be better to test for disease antigens rather than for antibodies. * * * * * * * 10. Home pregnancy tests utilize ELISA technology. When a woman is pregnant, her body produces a hormone called human chorionic gonadotropin (hCG). Explain how antibodies can
Activity 1.1.5: ELISA Name _______________________Per_____ |
Summarize the steps you took to conduct the ELISA test.
Conclusion 1. Explain why antibodies allow scientists to target and identify specific disease agents. * * * * * * 2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate? * * * * * 3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100? * * * * 4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate. * * * * * * 5. Why do you think college students living in dorms are often populations who see meningitis outbreaks? * * * * * 6. How did ELISA data allow you to track the path of infection at the college? * * * * * * 7. Discuss the limitations of using antigen concentration to deduce the path of infection. Be sure to refer to the workings of the human immune system. * * * * * * 8. The ELISA test can also be used to detect antibodies that are produced in response to a specific antigen. Using information about how you completed this ELISA experiment, outline a procedure for testing for antibodies in the blood. * * * * * * 9. Explain why in sudden outbreaks, it may be better to test for disease antigens rather than for antibodies. * * * * * * * 10. Home pregnancy tests utilize ELISA technology. When a woman is pregnant, her body produces a hormone called human chorionic gonadotropin (hCG). Explain how antibodies can