5.3.7 Effect Of Carbon Supplements
5.3.7 Effect of carbon supplements
Supplementation of sugars, which may act either as carbon sources or inducers,greatly affect the growth of microorganism.
In case of CMCase,out of the supplemented sugars,glucose,sucrose,maltose,lactose,wheatbran and CMC, wheat bran show maximum increase in enzyme production.
On addition of glucose, maltose, lactose,wheat bran and xylan, the maltose resulted in an increment in xylanase production (24.96 IU/ml) by SmF.
An induction in the enzyme production/activity was noted when fungal isolate (Aspergillus niger) was grown on cellulose as a sole carbon source while a very low rate of enzyme activity/production was observed when glucose was used as sole carbon substrate. These results are in agreement with …show more content…
In other words, they can be used with media replacements. Reuse numbers of immobilized A. niger was investigated in cmcase enzyme production.
The operational stability of the biocatalysts obtained under optimal immobilization conditions (alginate concentration 4%, 40 beads/flask) was followed during 4 cycles (fig….) to investigate the stability of biocatalyst and its ability to produce cmcases. cmcases were produced in repeated batch shake cultures and the time for each batch was 48h. When the estimated maximum activity level had been reached, the culture supernatant was decanted off and 20 mL of fresh medium was added to the Erlenmeyer flasks. The results obtained at the end of the first cycle showed a significant increase in cmcases activity of entrapped cells compared to those with free cells cultured in parallel. The immobilized cells could be reused effectively for enzyme production three times with a loss of 40% in the enzyme activity. Cmcase production decreased while re-use number increased. This behaviour could be attributed to clogging of the pores of the A. niger immobilized with Ca alginate gel. This reusability of this experiment is in accordance with the findings reported by other authors (Hemchander et al., 2001; Angelova et al., 1998; Ellaiah et al., 2004; Slokoska et al.,
Supplementation of sugars, which may act either as carbon sources or inducers,greatly affect the growth of microorganism.
In case of CMCase,out of the supplemented sugars,glucose,sucrose,maltose,lactose,wheatbran and CMC, wheat bran show maximum increase in enzyme production.
On addition of glucose, maltose, lactose,wheat bran and xylan, the maltose resulted in an increment in xylanase production (24.96 IU/ml) by SmF.
An induction in the enzyme production/activity was noted when fungal isolate (Aspergillus niger) was grown on cellulose as a sole carbon source while a very low rate of enzyme activity/production was observed when glucose was used as sole carbon substrate. These results are in agreement with …show more content…
In other words, they can be used with media replacements. Reuse numbers of immobilized A. niger was investigated in cmcase enzyme production.
The operational stability of the biocatalysts obtained under optimal immobilization conditions (alginate concentration 4%, 40 beads/flask) was followed during 4 cycles (fig….) to investigate the stability of biocatalyst and its ability to produce cmcases. cmcases were produced in repeated batch shake cultures and the time for each batch was 48h. When the estimated maximum activity level had been reached, the culture supernatant was decanted off and 20 mL of fresh medium was added to the Erlenmeyer flasks. The results obtained at the end of the first cycle showed a significant increase in cmcases activity of entrapped cells compared to those with free cells cultured in parallel. The immobilized cells could be reused effectively for enzyme production three times with a loss of 40% in the enzyme activity. Cmcase production decreased while re-use number increased. This behaviour could be attributed to clogging of the pores of the A. niger immobilized with Ca alginate gel. This reusability of this experiment is in accordance with the findings reported by other authors (Hemchander et al., 2001; Angelova et al., 1998; Ellaiah et al., 2004; Slokoska et al.,