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Amphotericin B Lab Report

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Amphotericin B Lab Report
Preformulation activities range from supporting identification of new active agents to characterizing physical properties necessary for the design of dosage forms. Critical information provided during the preformulation can enhance the rapid and successful introduction of new therapeutic entities for humans. Hence, preformulation studies are essential to characterize drugs for proper designing of drug delivery systems. The preformulation studies, which were performed in this project, include – identification of drug (physical appearance, chemical tests, IR spectra and UV absorption maxima), quantitative solubility studies and estimation of drug. The drug Amphotericin B was obtained as a gift sample for the present study.
5.1 PHYSICAL APPEARANCE
The drug sample of Amphotericin B supplied by Life Innovation Care, (H.P.) as gift sample was Yellow colored, odorless crystalline powder (Table 5.1).
5.2 DRUG IDENTIFICATION
Amphotericin B was identified by the procedures described in British Pharmacopoeia 2003.
5.2.1 Chemical Test
In a drug solution (1 mL of 0.05% w/v) in dimethyl sulfoxide (DMSO), 5 mL of orthophosphoric acid was added. A blue ring was observed at the
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The volume was made to 10 mL with methanol. One mL of the above solution was diluted to 100 times in a 100 mL volumetric flask with methanol to produce stock solution of 10 g/mL. From this solution 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 mL were withdrawn separately in different 10 mL volumetric flasks and volume was made up in each case up to 10mL with methanol to produce the concentrations 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 g/mL. Absorbances of these solutions were recorded at max 405.9 nm against methanol as blank using Shimadzu (1601) UV/Visible spectrophotometer, which is recorded in table 5.4. Standard curve is presented in figure

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