Anti Epilepsys Research Paper
VARIOUS IN VIVO MODELS FOR EVALUATION OF ANTI EPILEPTIC DRUGS :
The development of various new antiepileptic drugs (AEDs) in recent decades, the search for new therapies with better efficacy and tolerability remains an important goal[1]. The discovery and development of a new AED relies heavily on the preclinical use of animal models to establish efficacy and safety prior to first trials in humans[2].
ELECTRICALLY INDUCED SEIZURES :
Electroshock seizures in mice and rats:
Merritt and Putnam (1938) developed the MES test and discovered the convulsive effect of diphenylhydantoin using this test[3]. Protection against electroshock induced seizures in mice and rats is used as an indication for compounds which may prove beneficial in epilepsy. The electrical stimulus evokes hind limb extension which can be suppressed by a given dose of anti epileptic medications.
The resultant seizures passes through various phase: phase of tonic limb flexion of about 1.5 sec duration followed by phase of tonic limb extension lasting about 10 sec and finally followed by a variable short clonic interval which may lead to asphyxia death in some animals[4].
Suppression of tonic hind limb extension is taken as a measure the efficacy in this test. Drug effective …show more content…
In rats the electrode is implanted in the right amygdale for electrical stimulation. Animal is allowed to recover from surgery for a minimum of 1-2 weeks; otherwise the sensitivity of the animals to kindling is lowered. Then daily electrical stimulus trains are applied via the electrode using either a fixed current strength (400-500 μA, 1 msec monophase square wave pulses for 1 sec with 50 or 60/sec frequency) or using the individual threshold current to induce after discharge at the site of
The development of various new antiepileptic drugs (AEDs) in recent decades, the search for new therapies with better efficacy and tolerability remains an important goal[1]. The discovery and development of a new AED relies heavily on the preclinical use of animal models to establish efficacy and safety prior to first trials in humans[2].
ELECTRICALLY INDUCED SEIZURES :
Electroshock seizures in mice and rats:
Merritt and Putnam (1938) developed the MES test and discovered the convulsive effect of diphenylhydantoin using this test[3]. Protection against electroshock induced seizures in mice and rats is used as an indication for compounds which may prove beneficial in epilepsy. The electrical stimulus evokes hind limb extension which can be suppressed by a given dose of anti epileptic medications.
The resultant seizures passes through various phase: phase of tonic limb flexion of about 1.5 sec duration followed by phase of tonic limb extension lasting about 10 sec and finally followed by a variable short clonic interval which may lead to asphyxia death in some animals[4].
Suppression of tonic hind limb extension is taken as a measure the efficacy in this test. Drug effective …show more content…
In rats the electrode is implanted in the right amygdale for electrical stimulation. Animal is allowed to recover from surgery for a minimum of 1-2 weeks; otherwise the sensitivity of the animals to kindling is lowered. Then daily electrical stimulus trains are applied via the electrode using either a fixed current strength (400-500 μA, 1 msec monophase square wave pulses for 1 sec with 50 or 60/sec frequency) or using the individual threshold current to induce after discharge at the site of