Aseptic Technique and Bacterial Anatomy and Morphology
Lab, Week
ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY
Introduction
Part I: Aseptic Technique
The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures.
In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining to his doctor about pain in his lower abdominal and back. With a family history of prostate cancer, he worried that his sudden need to urinate during the night and infrequent appearance of blood in his urine might be bad signs. An ultrasound scan determined that the prostate gland was swollen. The physician sent a urine specimen to the lab for analysis. This organism was detected in large numbers in the urine.
After performing the aseptic techniques, it was found that if the proper techniques are not performed within a certain time frame the specimen will become contaminated.
Part II: Bacterial Anatomy
The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques.
Sample 1
In this study a female patient who had a problem that had arisen suddenly after lunch, had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she was violently allergic to wheat gluten, but the waiter had assured her that the meal was wheat gluten-free. Her lawyer-husband had his investigators at the restaurant before they closed that night, testing for any evidence that would uncover the cause. This is one of the organisms that the investigators found in the dishes she had that day.
A sample of the unknown organism was used to perform a gram stain procedure.
Sample 2
In this study a dead goat was found in
ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY
Introduction
Part I: Aseptic Technique
The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures.
In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining to his doctor about pain in his lower abdominal and back. With a family history of prostate cancer, he worried that his sudden need to urinate during the night and infrequent appearance of blood in his urine might be bad signs. An ultrasound scan determined that the prostate gland was swollen. The physician sent a urine specimen to the lab for analysis. This organism was detected in large numbers in the urine.
After performing the aseptic techniques, it was found that if the proper techniques are not performed within a certain time frame the specimen will become contaminated.
Part II: Bacterial Anatomy
The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques.
Sample 1
In this study a female patient who had a problem that had arisen suddenly after lunch, had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she was violently allergic to wheat gluten, but the waiter had assured her that the meal was wheat gluten-free. Her lawyer-husband had his investigators at the restaurant before they closed that night, testing for any evidence that would uncover the cause. This is one of the organisms that the investigators found in the dishes she had that day.
A sample of the unknown organism was used to perform a gram stain procedure.
Sample 2
In this study a dead goat was found in