BSL-1 Biosafety Level 1
Biosafety Level 1 BSL-1 is appropriate for working with microorganisms that are not known to cause disease in healthy human humans. This is the type of laboratory found in municipal water-testing laboratories, in high schools, and in some community colleges teaching introductory microbiology classes, where the agents are not considered hazardous. There is a door that can be closed to keep visitors out of the lab while work with the agents is in progress. Hazard warning signs may be posted on the door indicating any hazards that may be present, including radioactive materials, lazar lights, high noise emitting equipment, or toxic chemicals. There is a hand-washing sink available, preferably near the door. Waste materials are segregated according to hazard
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type, and there is an appropriate chemical decon tray for collecting contaminated implements. Work is done on the open bench, and plastic-backed absorbent pads can be placed on the work surface to collect splatter or droplets associated with the work.
The bench tops should be impervious to acid and all furniture should be sturdy. If there are openable windows in the lab, they should be fitted with screens. The lab should be constructed in such a manner that it can be easily cleaned and decontaminated. At BSL-1 there is no specific recommendation that the laboratory be isolated from other parts of the building. Although there is no specific biological safety reason for having more than six air changes per hour in a BSL-1 laboratory, it may be necessary if there are volatile or toxic chemicals in use. In general, inward directional airflow is the ideal. standard microbiological practices include the use of mechanical pipetting devices, having a prohibition on eating, drinking and smoking in the lab, and requiring hand washing by all persons when they finish their work or when exiting the laboratory. Persons working in the lab should wear a lab coat to protect their street clothes. It is a recommended practice to wear gloves while manipulating the agents. Additional protective equipment may include working behind a splatter shield or wearing eye or face protection. At BSL-1, no special precautions are
needed. Hand washing is one of the most important procedures that can be used by laboratorians to prevent removal of unwanted microbiological agents, radioactive materials, or chemicals from the laboratory environment. Use of liquid soap is generally preferable to bar soap; twenty seconds of vigorous lathering will remove most of these materials very effectively. After drying your hands with a paper towel, you can use the towel to turn off the faucets and thus prevent recontaminating your hands. The scientist who provides overall supervision to a BSL-1 laboratory needs to have general training in microbiology or a related science. The supervisor is responsible for establishing the general lab safety procedures and for ensuring that each laboratorian is properly educated in these procedures. Lab personnel, on the other hand, need to accept such training and follow the proscribed protocols.
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type, and there is an appropriate chemical decon tray for collecting contaminated implements. Work is done on the open bench, and plastic-backed absorbent pads can be placed on the work surface to collect splatter or droplets associated with the work.
The bench tops should be impervious to acid and all furniture should be sturdy. If there are openable windows in the lab, they should be fitted with screens. The lab should be constructed in such a manner that it can be easily cleaned and decontaminated. At BSL-1 there is no specific recommendation that the laboratory be isolated from other parts of the building. Although there is no specific biological safety reason for having more than six air changes per hour in a BSL-1 laboratory, it may be necessary if there are volatile or toxic chemicals in use. In general, inward directional airflow is the ideal. standard microbiological practices include the use of mechanical pipetting devices, having a prohibition on eating, drinking and smoking in the lab, and requiring hand washing by all persons when they finish their work or when exiting the laboratory. Persons working in the lab should wear a lab coat to protect their street clothes. It is a recommended practice to wear gloves while manipulating the agents. Additional protective equipment may include working behind a splatter shield or wearing eye or face protection. At BSL-1, no special precautions are
needed. Hand washing is one of the most important procedures that can be used by laboratorians to prevent removal of unwanted microbiological agents, radioactive materials, or chemicals from the laboratory environment. Use of liquid soap is generally preferable to bar soap; twenty seconds of vigorous lathering will remove most of these materials very effectively. After drying your hands with a paper towel, you can use the towel to turn off the faucets and thus prevent recontaminating your hands. The scientist who provides overall supervision to a BSL-1 laboratory needs to have general training in microbiology or a related science. The supervisor is responsible for establishing the general lab safety procedures and for ensuring that each laboratorian is properly educated in these procedures. Lab personnel, on the other hand, need to accept such training and follow the proscribed protocols.
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