Bacteria Gram Stain Lab Report
Microbiology 197
Prepared Bacteria Gram Stains (F12)
Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli
Procedure:
1. Observe each of slides listed in “Specimens” above.
2. Make your observations using oil immersion (1000X).
3. Using a drawing form draw the organisms you see using proper scale and proportion.
4. Provide the following “Observations” in the space to the right of the drawing circle: a. Gram reaction (color and interpretation)
b. Morphology (shape)
c. Arrangement (how did the bacterial …show more content…
How will a microbiologist use this staining method to begin the identification of bacteria?
Microbiologists use this staining method to begin the identification of bacteria because it will allow them to tell the four different categories based upon color reaction and shape. It is the first step that will help in identifying bacteria. The structural variations found in the cell walls of bacteria will determine the color of the stain; blue (positive) or red (negative).
2. Give one specific clinical reason for gram staining a patient's sputum specimen.
One specific clinical reason for gram staining a patient’s sputum specimen would be to isolate and identify microorganisms causing an infection of the lower respiratory tract. Examples would be tuberculosis or bacterial pneumonia. A gram stain is always performed when sputum is submitted for culture and used to determine the adequacy of the specimen for culture. This aids the microbiologist in selecting special growth media that might be needed. Almost all bacteria are described by their gram stain characteristics: color, shape and size, and arrangement. 3. For each of the three slides: Did the organism appear as described by the instructor for: 1. gram reaction, 2. morphology, and 3.
Prepared Bacteria Gram Stains (F12)
Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli
Procedure:
1. Observe each of slides listed in “Specimens” above.
2. Make your observations using oil immersion (1000X).
3. Using a drawing form draw the organisms you see using proper scale and proportion.
4. Provide the following “Observations” in the space to the right of the drawing circle: a. Gram reaction (color and interpretation)
b. Morphology (shape)
c. Arrangement (how did the bacterial …show more content…
How will a microbiologist use this staining method to begin the identification of bacteria?
Microbiologists use this staining method to begin the identification of bacteria because it will allow them to tell the four different categories based upon color reaction and shape. It is the first step that will help in identifying bacteria. The structural variations found in the cell walls of bacteria will determine the color of the stain; blue (positive) or red (negative).
2. Give one specific clinical reason for gram staining a patient's sputum specimen.
One specific clinical reason for gram staining a patient’s sputum specimen would be to isolate and identify microorganisms causing an infection of the lower respiratory tract. Examples would be tuberculosis or bacterial pneumonia. A gram stain is always performed when sputum is submitted for culture and used to determine the adequacy of the specimen for culture. This aids the microbiologist in selecting special growth media that might be needed. Almost all bacteria are described by their gram stain characteristics: color, shape and size, and arrangement. 3. For each of the three slides: Did the organism appear as described by the instructor for: 1. gram reaction, 2. morphology, and 3.