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Colony Morphology

Color- light yellow pigmentation
Form- circular
Elevation- convex
Margin- entire
[staphylococci]

DNase Test

This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result.

Blood agar plate

This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my bacteria no change is observed in the media therefore the bacteria is non-hemolytic and negative result.

MacConkey Agar test

This test is used for isolating the lactose fermenting bacteria from lactose non-fermenting bacteria while inhibiting the growth of gram positive bacteria.
The lack of growth from my bacteria indicates that my bacterium has negative result.

Mannitol Salt Agar

This test is used to observe the difference between gram positive staphylococci bacteria and to differentiate for mannitol fermentation. If a yellow media change is observed then the bacteria has the presence of acid by-products of mannitol fermentation. For my bacteria no change in the color of the media is observed but growth is. Therefore we know that our bacteria has no mannitol fermentation.

Gram Stain

This test is performed to get a description of the make-up of the cell membrane of the bacteria. A gram positive bacteria will display a blue/violet pigmentation where a gram negative bacteria will display a pink/red pigmentation. This is due to the outer membrane of gram negative bacteria. My unknown bacteria displays a pink color hinting the presence of a gram negative bacteria.

Citrate Agar Slant

This test is


References: 1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655438/ 2. http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2002.03183.x/full 3. http://link.springer.com/article/10.1007/s00253-012-4539-5 4. Baertsch R, Lowe T, Pohl A, Pollard K, Schneider K. 2001. Staphylococcus aureus Mu50 (Staphylococcus aureus subsp. aureus Mu50) Genome Browser Gateway. [Online]. Available from: http://microbes.ucsc.edu/cgi-bin/hgGateway?db=stapAure_MU50. Accessed 2013 Mar 22. 5. Gilichinsky D, Goris J, Rodrigues D, Thomashow M, Tiedje J, Vishnivetskaya T. 2006 Aug. Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. [Online]. Available from: http://link.springer.com/article/10.1007%2Fs00792-005-0497-5. Accessed 2013 Mar 22. 6. Unknown. 2006 Mar 29. Staphylococcal Food Poisoning. [Online]. Available from: http://www.cdc.gov/ncidod/dbmd/diseaseinfo/staphylococcus_food_g.htm. Accessed 2013 Mar 22.
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