Beers law
Determining the Concentration of a solution: Beer's Law
Objective
In this lab of Determining the concentration of a unknown solution: Beers Law. We determined the concentration of a unknown CuSO4 solution by measuring its absorbance with the colorimeter. With all the calculations we were able to solve the linear regression Equation of absorbance vs. concentration and the alternate method.
Materials
Vernier LabPro or CBL 2 interface .40 M CuSO4 solution
Computer or handheld CuSO4 unknown solution
Vernier Colorimeter Pipet pump or pump bulb one cuvette Distilled water five 20*150 mm test tubes test tube rack two 10 mL pipets or graduated cylinders stirring rod two 100 mL beakers procedures 1. Obtain and wear goggles. 2. Obtain small volumes of 0.40 M CuSO4 solution and distilled water in separate beakers. 3. Label four clean, dry, test tubes 1–4. Use pipets to prepare five standard solutions according to the chart below. Thoroughly mix each solution with a stirring rod. Clean and dry the stirring rod between uses.
Trail number
0.40 M CuSo4 (mL)
Distilled H2O (mL)
Concentration (M)
1
2
8
0.08
2
4
6
0.16
3
6
4
0.24
4
8
2
0.32
5
10
0
0.40
4.Set up the data collection system. Connect the LabPro or CBL 2 interface to the computer or handheld with the proper cable.
a) Connect a colorimeter to channel 1 of the interface
b) Start the data collection program. Be sure the program shows correct reading for the colorimeter.
c) Set up data collection for events with entry.
d) Follow the appropriate steps to calibrate the colorimeter.
5. Collect and record the absorbance values for each of the five standards solutions. Print a graph of absorbance vs. concentration.
6.Collect and record the absorbance value for the unknown solution that you
Objective
In this lab of Determining the concentration of a unknown solution: Beers Law. We determined the concentration of a unknown CuSO4 solution by measuring its absorbance with the colorimeter. With all the calculations we were able to solve the linear regression Equation of absorbance vs. concentration and the alternate method.
Materials
Vernier LabPro or CBL 2 interface .40 M CuSO4 solution
Computer or handheld CuSO4 unknown solution
Vernier Colorimeter Pipet pump or pump bulb one cuvette Distilled water five 20*150 mm test tubes test tube rack two 10 mL pipets or graduated cylinders stirring rod two 100 mL beakers procedures 1. Obtain and wear goggles. 2. Obtain small volumes of 0.40 M CuSO4 solution and distilled water in separate beakers. 3. Label four clean, dry, test tubes 1–4. Use pipets to prepare five standard solutions according to the chart below. Thoroughly mix each solution with a stirring rod. Clean and dry the stirring rod between uses.
Trail number
0.40 M CuSo4 (mL)
Distilled H2O (mL)
Concentration (M)
1
2
8
0.08
2
4
6
0.16
3
6
4
0.24
4
8
2
0.32
5
10
0
0.40
4.Set up the data collection system. Connect the LabPro or CBL 2 interface to the computer or handheld with the proper cable.
a) Connect a colorimeter to channel 1 of the interface
b) Start the data collection program. Be sure the program shows correct reading for the colorimeter.
c) Set up data collection for events with entry.
d) Follow the appropriate steps to calibrate the colorimeter.
5. Collect and record the absorbance values for each of the five standards solutions. Print a graph of absorbance vs. concentration.
6.Collect and record the absorbance value for the unknown solution that you